OBJECTIVE: To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation. DESIGN: The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined. SETTING: Private hospital-based fertility center and IVF program. MAIN OUTCOME MEASURE(S): Percentage of embryos continuing cell division and implantation rates after ET. RESULT(S): Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation. CONCLUSION(S): Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.
OBJECTIVE: To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation. DESIGN: The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined. SETTING: Private hospital-based fertility center and IVF program. MAIN OUTCOME MEASURE(S): Percentage of embryos continuing cell division and implantation rates after ET. RESULT(S): Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation. CONCLUSION(S): Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.
Authors: Mohamed A Bedaiwy; Tommaso Falcone; Jeffrey M Goldberg; Marjan Attaran; Rakesh Sharma; Kurt Miller; David R Nelson; Ashok Agarwal Journal: J Assist Reprod Genet Date: 2005-04 Impact factor: 3.412