Literature DB >> 9339471

Electroporation-mediated transformation of Arcanobacterium (Actinomyces) pyogenes.

B H Jost1, S J Billington, J G Songer.   

Abstract

Plasmids derived from pNG2 or RSF1010 were introduced into strains of Arcanobacterium (Actinomyces) pyogenes by electroporation. Electroporation conditions were varied systematically to give a maximum electroporation frequency of 3.7 x 10(5) CFU/microgram DNA at 1.5 kV/cm and 246 omega, resulting in a time constant of approximately 10 ms. The A. pyogenes transformants expressed plasmid-encoded resistance to chloramphenicol, erythromycin, kanamycin, and streptomycin. The source of incoming DNA affected the growth rate of transformants, but not the electroporation efficiency. This is the first report of genetic transformation of the veterinary pathogen A. pyogenes.

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Mesh：

Year:  1997        PMID： 9339471     DOI： 10.1006/plas.1997.1299

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  8 in total

1.  An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.

Authors:  B H Jost; J G Songer; S J Billington
Journal:  Infect Immun       Date:  1999-04       Impact factor: 3.441

2.  The Arcanobacterium (Actinomyces) pyogenes plasmid pAP1 is a member of the pIJ101/pJV1 family of rolling circle replication plasmids.

Authors:  S J Billington; B H Jost; J G Songer
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

3.  Cloning, expression, and characterization of a neuraminidase gene from Arcanobacterium pyogenes.

Authors:  B H Jost; J G Songer; S J Billington
Journal:  Infect Immun       Date:  2001-07       Impact factor: 3.441

4.  Immunization with genetic toxoids of the Arcanobacterium pyogenes cholesterol-dependent cytolysin, pyolysin, protects mice against infection.

Authors:  B Helen Jost; Hien T Trinh; J Glenn Songer; Stephen J Billington
Journal:  Infect Immun       Date:  2003-05       Impact factor: 3.441

5.  Identification of a second Arcanobacterium pyogenes neuraminidase and involvement of neuraminidase activity in host cell adhesion.

Authors:  B Helen Jost; J Glenn Songer; Stephen J Billington
Journal:  Infect Immun       Date:  2002-03       Impact factor: 3.441

6.  Phospholipase D promotes Arcanobacterium haemolyticum adhesion via lipid raft remodeling and host cell death following bacterial invasion.

Authors:  Erynn A Lucas; Stephen J Billington; Petteri Carlson; David J McGee; B Helen Jost
Journal:  BMC Microbiol       Date:  2010-10-25       Impact factor: 3.605

7.  Tylosin resistance in Arcanobacterium pyogenes is encoded by an erm X determinant.

Authors:  B Helen Jost; Adam C Field; Hien T Trinh; J Glenn Songer; Stephen J Billington
Journal:  Antimicrob Agents Chemother       Date:  2003-11       Impact factor: 5.191

8.  The Arcanobacterium pyogenes collagen-binding protein, CbpA, promotes adhesion to host cells.

Authors:  Paula A Esmay; Stephen J Billington; Malen A Link; J Glenn Songer; B Helen Jost
Journal:  Infect Immun       Date:  2003-08       Impact factor: 3.441
  8 in total

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