Literature DB >> 9336851

Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A.

K K Kim1, H Yokota, R Kim, S H Kim.   

Abstract

A gene coding for a protein homologous to a translation initiation factor of eukaryotes, eIF5A, was cloned from Methanococcus jannaschii, a hyperthermophile with an optimum growth temperature of 85 degrees C. The protein was overexpressed, purified and crystallized. The crystals were obtained by vapor diffusion method with 8% PEG 4000 as precipitant and belong to space group P4(1)22 with unit cell dimensions a = b = 45.52 A and c = 155.59 A. These crystals diffract to at least 2.2 A resolution.

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Year:  1997        PMID： 9336851      PMCID： PMC2143566          DOI： 10.1002/pro.5560061023

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  8 in total

1.  Effect of initiation factor eIF-5A depletion on protein synthesis and proliferation of Saccharomyces cerevisiae.

Authors:  H A Kang; J W Hershey
Journal:  J Biol Chem       Date:  1994-02-11       Impact factor: 5.157

2.  Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta.

Authors:  W M Kemper; K W Berry; W C Merrick
Journal:  J Biol Chem       Date:  1976-09-25       Impact factor: 5.157

3.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

4.  Hypusine formation: a unique posttranslational modification in translation initiation factor eIF-4D.

Authors:  H L Cooper; M H Park; J E Folk
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

5.  Effects of translation initiation factor eIF-5A on the functioning of human T-cell leukemia virus type I Rex and human immunodeficiency virus Rev inhibited trans dominantly by a Rex mutant deficient in RNA binding.

Authors:  J Katahira; T Ishizaki; H Sakai; A Adachi; K Yamamoto; H Shida
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103

6.  Translation initiation factor 5A and its hypusine modification are essential for cell viability in the yeast Saccharomyces cerevisiae.

Authors:  J Schnier; H G Schwelberger; Z Smit-McBride; H A Kang; J W Hershey
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

7.  Inhibition of the G1-S transition of the cell cycle by inhibitors of deoxyhypusine hydroxylation.

Authors:  H M Hanauske-Abel; M H Park; A R Hanauske; A M Popowicz; M Lalande; J E Folk
Journal:  Biochim Biophys Acta       Date:  1994-03-31

8.  Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation.

Authors:  M Ruhl; M Himmelspach; G M Bahr; F Hammerschmid; H Jaksche; B Wolff; H Aschauer; G K Farrington; H Probst; D Bevec
Journal:  J Cell Biol       Date:  1993-12       Impact factor: 10.539
  8 in total
  5 in total

1.  Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8 A resolution.

Authors:  K K Kim; L W Hung; H Yokota; R Kim; S H Kim
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

2.  Chemical denaturation and elevated folding temperatures are required for wild-type activity and stability of recombinant Methanococcus jannaschii 20S proteasome.

Authors:  R J Frankenberg; T S Hsu; H Yakota; R Kim; D S Clark
Journal:  Protein Sci       Date:  2001-09       Impact factor: 6.725

3.  Inhibition of cell growth through inactivation of eukaryotic translation initiation factor 5A (eIF5A) by deoxyspergualin.

Authors:  Kazuhiro Nishimura; Yuji Ohki; Tomomi Fukuchi-Shimogori; Kaori Sakata; Kan Saiga; Takanobu Beppu; Akira Shirahata; Keiko Kashiwagi; Kazuei Igarashi
Journal:  Biochem J       Date:  2002-05-01       Impact factor: 3.857

4.  Oxazolidinone antibiotics target the P site on Escherichia coli ribosomes.

Authors:  Hiroyuki Aoki; Lizhu Ke; Susan M Poppe; Toni J Poel; Elizabeth A Weaver; Robert C Gadwood; Richard C Thomas; Dean L Shinabarger; M Clelia Ganoza
Journal:  Antimicrob Agents Chemother       Date:  2002-04       Impact factor: 5.191

5.  Inhibition of Eukaryotic Translation Initiation Factor 5A (eIF5A) Hypusination Suppress p53 Translation and Alters the Association of eIF5A to the Ribosomes.

Authors:  Marianna Martella; Caterina Catalanotto; Claudio Talora; Anna La Teana; Paola Londei; Dario Benelli
Journal:  Int J Mol Sci       Date:  2020-06-27       Impact factor: 5.923
  5 in total

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