Rapid PCR amplification of chimeric products and its direct application to in vivo [corrected] testing of recombinant DNA construction strategies.

This article describes a simple and rapid method for efficient production of chimeric products by polymerase chain reaction (PCR). This protocol is amenable to site-directed mutagenesis strategies and can be done without the time-consuming gel purification step. The PCR products generated can also be directly used for direct gene transfer into plant cells without further subcloning to test construction strategies.