BACKGROUND: We studied the effect of FK506 ointment on rat skin allograft survival. METHODS: Lewis rat (RT1(1)) skin allografts were histologically evaluated on day 7 after transplantation to recipient ACI (RT1a) rats. We set histological gradings for rejection as follows: grade 1, intraepidermal blister formation; grade 2, incomplete epidermal separation from the dermis; and grade 3, complete epidermal separation from the epidermis. According to these histological criteria, the immunosuppressive effect of FK506 ointment was assessed. RESULTS: Our data indicated that all recipient rats without FK506 ointment showed grade 3 rejection on day 7 after transplantation. In contrast, the allografts treated with FK506 ointment showed grade 1 rejection. Furthermore the blood concentration of FK506 was below 0.5 ng/ml, minimizing the systemically adverse effects of FK506. CONCLUSIONS: Thus, the present study suggests that the topical administration of FK506 on the skin allografts may be useful and effective in the suppression of skin allograft rejection.
BACKGROUND: We studied the effect of FK506 ointment on rat skin allograft survival. METHODS: Lewis rat (RT1(1)) skin allografts were histologically evaluated on day 7 after transplantation to recipient ACI (RT1a) rats. We set histological gradings for rejection as follows: grade 1, intraepidermal blister formation; grade 2, incomplete epidermal separation from the dermis; and grade 3, complete epidermal separation from the epidermis. According to these histological criteria, the immunosuppressive effect of FK506 ointment was assessed. RESULTS: Our data indicated that all recipient rats without FK506 ointment showed grade 3 rejection on day 7 after transplantation. In contrast, the allografts treated with FK506 ointment showed grade 1 rejection. Furthermore the blood concentration of FK506 was below 0.5 ng/ml, minimizing the systemically adverse effects of FK506. CONCLUSIONS: Thus, the present study suggests that the topical administration of FK506 on the skin allografts may be useful and effective in the suppression of skin allograft rejection.
Authors: Melissa Mastroianni; Zhi Yang Ng; Ritu Goyal; Christopher Mallard; Evan A Farkash; David A Leonard; Alexander Albritton; Kumaran Shanmugarajah; Josef M Kurtz; David H Sachs; Lauren K Macri; Joachim Kohn; Curtis L Cetrulo Journal: J Burn Care Res Date: 2018-04-20 Impact factor: 1.845
Authors: Poulami Majumder; Yichuan Zhang; Marcos Iglesias; Lixin Fan; James A Kelley; Caroline Andrews; Nimit Patel; Jason R Stagno; Byoung Chol Oh; Georg J Furtmüller; Christopher C Lai; Yun-Xing Wang; Gerald Brandacher; Giorgio Raimondi; Joel P Schneider Journal: Small Date: 2020-08-18 Impact factor: 15.153