Literature DB >> 932560

Isolation of disaturated phosphatidylcholine with osmium tetroxide.

R J Mason, J Nellenbogen, J A Clements.   

Abstract

A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.

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Year:  1976        PMID: 932560

Source DB:  PubMed          Journal:  J Lipid Res        ISSN: 0022-2275            Impact factor:   5.922


  121 in total

1.  Effect of hyperpnea on enzymes of the CDPcholine path for phosphatidylcholine synthesis in rat lung.

Authors:  H A Barr; T E Nicholas; J H Power
Journal:  Lipids       Date:  1989-08       Impact factor: 1.880

2.  Stat-3 is required for pulmonary homeostasis during hyperoxia.

Authors:  Isamu Hokuto; Machiko Ikegami; Mitsuhiro Yoshida; Kiyoshi Takeda; Shizuo Akira; Anne-Karina T Perl; William M Hull; Susan E Wert; Jeffrey A Whitsett
Journal:  J Clin Invest       Date:  2004-01       Impact factor: 14.808

3.  Role of lung fluid volume in growth and maturation of the fetal sheep lung.

Authors:  A C Moessinger; R Harding; T M Adamson; M Singh; G T Kiu
Journal:  J Clin Invest       Date:  1990-10       Impact factor: 14.808

4.  Surfactant protein composition of lamellar bodies isolated from rat lung.

Authors:  M A Oosterlaken-Dijksterhuis; M van Eijk; B L van Buel; L M van Golde; H P Haagsman
Journal:  Biochem J       Date:  1991-02-15       Impact factor: 3.857

5.  Nebulization of Poractant alfa via a vibrating membrane nebulizer in spontaneously breathing preterm lambs with binasal continuous positive pressure ventilation.

Authors:  Matthias C Hütten; Elke Kuypers; Daan R Ophelders; Maria Nikiforou; Reint K Jellema; Hendrik J Niemarkt; Carola Fuchs; Markus Tservistas; Roberta Razetti; Federico Bianco; Boris W Kramer
Journal:  Pediatr Res       Date:  2015-08-31       Impact factor: 3.756

Review 6.  Exogenous surfactant treatments for neonatal respiratory distress syndrome and their potential role in the adult respiratory distress syndrome.

Authors:  T A Merritt; M Hallman; R Spragg; G P Heldt; N Gilliard
Journal:  Drugs       Date:  1989-10       Impact factor: 9.546

7.  Recruited inflammatory cells mediate endotoxin-induced lung maturation in preterm fetal lambs.

Authors:  Suhas G Kallapur; Timothy J M Moss; Machiko Ikegami; Richard L Jasman; John P Newnham; Alan H Jobe
Journal:  Am J Respir Crit Care Med       Date:  2005-08-18       Impact factor: 21.405

8.  Betamethasone for lung maturation: testing dose and formulation in fetal sheep.

Authors:  Alan H Jobe; Timothy J M Moss; Ilias Nitsos; Machiko Ikegami; Suhas G Kallapur; John P Newnham
Journal:  Am J Obstet Gynecol       Date:  2007-11       Impact factor: 8.661

9.  Alveolar lavage and lavaged lung tissue phosphatidylcholine composition during fetal rabbit development.

Authors:  S A Rooney; L I Gobran
Journal:  Lipids       Date:  1977-12       Impact factor: 1.880

10.  Selective use of palmitic acid over stearic acid for synthesis of phosphatidylcholine and phosphatidylglycerol in lung.

Authors:  F H Tsao
Journal:  Lipids       Date:  1986-11       Impact factor: 1.880

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