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Literature DB >> 932559

An improved and simplified radioisotopic assay for the determination of free and esterified carnitine.

Abstract

The radioisotopic assay for carnitine first described by Cederblad and Lindstedt (Clin. Chim. Acta. 37:235-543, 1972) and modified by Bohmer et al (Clin. Chim. Acta. 57:55-61, 1974) has been improved and simplified. As a result, the assay yields a linear response over a wide range of carnitine concentrations without the need for excessive amounts of labeled acetyl-CoA. In addition, it will measure very small quantities of carnitine even in the presence of excess acylcarnitine. The method allows rapid determination of free and esterified carnitine in small volumes of plasma (50 mul is sufficient) without the need for prior deproteinization of the samples.

Entities: Chemical

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Year: 1976 PMID： 932559

Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922

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Cited

73 in total

1. Emetine-induced myopathy and carnitine deficiency.

Authors: T Kuntzer; H Reichmann; J Bogousslavsky; F Regli
Journal: J Neurol Date: 1990-12 Impact factor: 4.849

2. Subnormal carnitine levels and their correction in artificially fed patients from a neurological intensive care unit: a pilot study.

Authors: J Schäfer; H Reichmann
Journal: J Neurol Date: 1990-06 Impact factor: 4.849

3. Effect of propionyl-L-carnitine on mechanical function of isolated rabbit heart.

Authors: R Ferrari; E Pasini; E Condorelli; A Boraso; R Lisciani; A Marzo; O Visiou
Journal: Cardiovasc Drugs Ther Date: 1991-02 Impact factor: 3.727

4. Delineation of the influence of propionylcarnitine on the accumulation of long-chain acylcarnitines and electrophysiologic derangements evoked by hypoxia in canine myocardium.

Authors: K A Yamada; D J Dobmeyer; E M Kanter; S G Priori; P B Corr
Journal: Cardiovasc Drugs Ther Date: 1991-02 Impact factor: 3.727

An improved and simplified radioisotopic assay for the determination of free and esterified carnitine.

1. Emetine-induced myopathy and carnitine deficiency.

2. Subnormal carnitine levels and their correction in artificially fed patients from a neurological intensive care unit: a pilot study.

3. Effect of propionyl-L-carnitine on mechanical function of isolated rabbit heart.

4. Delineation of the influence of propionylcarnitine on the accumulation of long-chain acylcarnitines and electrophysiologic derangements evoked by hypoxia in canine myocardium.

5. Potential role of carnitine in patients with renal insufficiency.

6. Mitochondrial very long-chain acyl-CoA dehydrogenase deficiency with a mild clinical course.

7. Metabolism and disposition of intravenously administered acetyl-L-carnitine in healthy volunteers.

8. Glycine and L-carnitine therapy in 3-methylcrotonyl-CoA carboxylase deficiency.

9. Effects of carnitine in ischemic and fatty acid supplemented swine hearts.

10. Pharmacokinetics of oral acetyl-L-carnitine in renal impairment.