Literature DB >> 9324263

The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro.

C Disqué-Kochem1, B Dreiseikelmann.   

Abstract

The cytoplasmic protein TraM is one of four essential gene products of the F factor which are involved in DNA transfer after mating pair formation. TraM binds to three specific sites within the oriT region. Besides regulation of its own synthesis, the precise function of TraM during conjugation is not yet known. In the present work, the affinity of TraM to TraD was studied in vitro by an overlay assay and by affinity chromatography. Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed. A 35-kDa host membrane protein of yet unknown function also shows affinity to TraM and may be involved in this anchoring process as well.

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Year:  1997        PMID： 9324263      PMCID： PMC179519          DOI： 10.1128/jb.179.19.6133-6137.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  27 in total

Review 1.  Blotting and band-shifting: techniques for studying protein-protein interactions.

Authors:  D W Carr; J D Scott
Journal:  Trends Biochem Sci       Date:  1992-07       Impact factor: 13.807

2.  The TraM protein of plasmid R1 is a DNA-binding protein.

Authors:  M Schwab; H Gruber; G Högenauer
Journal:  Mol Microbiol       Date:  1991-02       Impact factor: 3.501

Review 3.  Analysis of the sequence and gene products of the transfer region of the F sex factor.

Authors:  L S Frost; K Ippen-Ihler; R A Skurray
Journal:  Microbiol Rev       Date:  1994-06

4.  Mutational and physical analysis of F plasmid traY protein binding to oriT.

Authors:  Y Luo; Q Gao; R C Deonier
Journal:  Mol Microbiol       Date:  1994-02       Impact factor: 3.501

Review 5.  Translocation of DNA across bacterial membranes.

Authors:  B Dreiseikelmann
Journal:  Microbiol Rev       Date:  1994-09

6.  Purification and properties of the F sex factor TraD protein, an inner membrane conjugal transfer protein.

Authors:  M M Panicker; E G Minkley
Journal:  J Biol Chem       Date:  1992-06-25       Impact factor: 5.157

7.  Characterization of the Escherichia coli F factor traY gene product and its binding sites.

Authors:  W C Nelson; B S Morton; E E Lahue; S W Matson
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490

8.  The TraM protein of the conjugative plasmid F binds to the origin of transfer of the F and ColE1 plasmids.

Authors:  L Di Laurenzio; L S Frost; W Paranchych
Journal:  Mol Microbiol       Date:  1992-10       Impact factor: 3.501

9.  Characterization of the oriT region of the IncFV plasmid pED208.

Authors:  L Di Laurenzio; L S Frost; B B Finlay; W Paranchych
Journal:  Mol Microbiol       Date:  1991-07       Impact factor: 3.501

10.  Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor.

Authors:  U Reygers; R Wessel; H Müller; H Hoffmann-Berling
Journal:  EMBO J       Date:  1991-09       Impact factor: 11.598
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  42 in total

1.  Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.

Authors:  M H Lee; N Kosuk; J Bailey; B Traxler; C Manoil
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

2.  Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation.

Authors:  K G Anthony; W A Klimke; J Manchak; L S Frost
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

3.  Genes for the type IV secretion system in an intracellular symbiont, Wolbachia, a causative agent of various sexual alterations in arthropods.

Authors:  S Masui; T Sasaki; H Ishikawa
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

Review 4.  The outs and ins of bacterial type IV secretion substrates.

Authors:  Zhiyong Ding; Krishnamohan Atmakuri; Peter J Christie
Journal:  Trends Microbiol       Date:  2003-11       Impact factor: 17.079

5.  TraG-like proteins of type IV secretion systems: functional dissection of the multiple activities of TraG (RP4) and TrwB (R388).

Authors:  Gunnar Schröder; Erich Lanka
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

6.  Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes.

Authors:  Matxalen Llosa; Sandra Zunzunegui; Fernando de la Cruz
Journal:  Proc Natl Acad Sci U S A       Date:  2003-08-18       Impact factor: 11.205

Review 7.  Mobility of plasmids.

Authors:  Chris Smillie; M Pilar Garcillán-Barcia; M Victoria Francia; Eduardo P C Rocha; Fernando de la Cruz
Journal:  Microbiol Mol Biol Rev       Date:  2010-09       Impact factor: 11.056

8.  The F-plasmid TraI protein contains three functional domains required for conjugative DNA strand transfer.

Authors:  Steven W Matson; Heather Ragonese
Journal:  J Bacteriol       Date:  2005-01       Impact factor: 3.490

9.  Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretion.

Authors:  Krishnamohan Atmakuri; Eric Cascales; Peter J Christie
Journal:  Mol Microbiol       Date:  2004-12       Impact factor: 3.501

10.  Characterization of the opposing roles of H-NS and TraJ in transcriptional regulation of the F-plasmid tra operon.

Authors:  William R Will; Laura S Frost
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490
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