OBJECTIVE: To study the in vitro effect of testosterone on anti-DNA antibody production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE) in order to elucidate its regulatory role in SLE. METHODS: PBMC from SLE patients were cultured with testosterone. IgG anti-double-stranded DNA (anti-dsDNA) antibody, total IgG, and cytokine activity in the supernatants were measured by enzyme-linked immunosorbent assay. RESULTS: Testosterone suppressed both IgG anti-dsDNA antibody and total IgG production in PBMC from SLE patients. Antibody production in B cells was also suppressed by testosterone, although the magnitude of its effect on B cells was lower than that on PBMC. Interleukin-6 (IL-6) partially restored the testosterone-induced decrease in antibody levels in PBMC. Testosterone reduced IL-6 production in monocytes. CONCLUSION: These results suggest that testosterone may directly suppress anti-DNA antibody production in PBMC from SLE patients by inhibiting B cell hyperactivity and, indirectly, by down-regulating IL-6 production in monocytes. These results support the therapeutic effects of testosterone on SLE.
OBJECTIVE: To study the in vitro effect of testosterone on anti-DNA antibody production in peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE) in order to elucidate its regulatory role in SLE. METHODS: PBMC from SLEpatients were cultured with testosterone. IgG anti-double-stranded DNA (anti-dsDNA) antibody, total IgG, and cytokine activity in the supernatants were measured by enzyme-linked immunosorbent assay. RESULTS:Testosterone suppressed both IgG anti-dsDNA antibody and total IgG production in PBMC from SLEpatients. Antibody production in B cells was also suppressed by testosterone, although the magnitude of its effect on B cells was lower than that on PBMC. Interleukin-6 (IL-6) partially restored the testosterone-induced decrease in antibody levels in PBMC. Testosterone reduced IL-6 production in monocytes. CONCLUSION: These results suggest that testosterone may directly suppress anti-DNA antibody production in PBMC from SLEpatients by inhibiting B cell hyperactivity and, indirectly, by down-regulating IL-6 production in monocytes. These results support the therapeutic effects of testosterone on SLE.
Authors: Michael P Corcoran; Mohsen Meydani; Alice H Lichtenstein; Ernst J Schaefer; Alice Dillard; Stefania Lamon-Fava Journal: J Endocrinol Date: 2010-05-18 Impact factor: 4.286
Authors: U Musabak; E Bolu; M Ozata; C Oktenli; A Sengul; A Inal; Z Yesilova; G Kilciler; I C Ozdemir; I H Kocar Journal: Clin Exp Immunol Date: 2003-05 Impact factor: 4.330
Authors: Piotr Piotrowski; Robert Gasik; Margarita Lianeri; Dorota Cieślak; Mariusz Wudarski; Paweł Hrycaj; Jan K Łacki; Paweł P Jagodziński Journal: Mol Biol Rep Date: 2009-08-01 Impact factor: 2.316