BACKGROUND & AIMS: Malnutrition is a complication of liver cirrhosis accompanied by reduced insulin-like growth factor I (IGF-I) availability. The aim of this study was to analyze the effect of IGF-I on intestinal D-galactose absorption in cirrhotic rats. METHODS: IGF-I (2 micrograms.100 g body wt-1.day-1) or saline were given for 14 days to rats in whom cirrhosis was induced with CCl4. Galactose transport and sodium-glucose/galactose-ligand transporter 1 (SGLT-1) expression were assessed in jejunal rings and in brush border membrane vesicles (BBMVs). RESULTS: Compared with that in controls, galactose transport in everted jejunal rings was significantly reduced in cirrhotic rats but showed normal values after IGF-I treatment. The kinetic study of D-galactose uptake by BBMVs showed decreased maximal velocity (Vmax) and diminished transporter affinity in cirrhotic rats. These kinetic parameters reverted to normal after IGF-I treatment. Microvilli were significantly elongated in cirrhotic rats but of normal size in the IGF-I-treated group. The expression of SGLT-1 on BBMVs (Western blot) and on the luminal membrane of enterocytes (immunohistochemistry) was not reduced in cirrhotic animals compared with controls or IGF-treated cirrhotic rats. CONCLUSIONS: Intestinal sugar transport is disturbed in experimental cirrhosis, and this alteration is corrected by IGF-I.
BACKGROUND & AIMS: Malnutrition is a complication of liver cirrhosis accompanied by reduced insulin-like growth factor I (IGF-I) availability. The aim of this study was to analyze the effect of IGF-I on intestinal D-galactose absorption in cirrhotic rats. METHODS:IGF-I (2 micrograms.100 g body wt-1.day-1) or saline were given for 14 days to rats in whom cirrhosis was induced with CCl4. Galactose transport and sodium-glucose/galactose-ligand transporter 1 (SGLT-1) expression were assessed in jejunal rings and in brush border membrane vesicles (BBMVs). RESULTS: Compared with that in controls, galactose transport in everted jejunal rings was significantly reduced in cirrhotic rats but showed normal values after IGF-I treatment. The kinetic study of D-galactose uptake by BBMVs showed decreased maximal velocity (Vmax) and diminished transporter affinity in cirrhotic rats. These kinetic parameters reverted to normal after IGF-I treatment. Microvilli were significantly elongated in cirrhotic rats but of normal size in the IGF-I-treated group. The expression of SGLT-1 on BBMVs (Western blot) and on the luminal membrane of enterocytes (immunohistochemistry) was not reduced in cirrhotic animals compared with controls or IGF-treated cirrhotic rats. CONCLUSIONS: Intestinal sugar transport is disturbed in experimental cirrhosis, and this alteration is corrected by IGF-I.
Authors: R Pérez; I Castilla-Cortázar; M Núñez; A Prado; E Mirpuri; M García; S González Barón; A Picardi Journal: J Physiol Biochem Date: 2001-03 Impact factor: 4.158
Authors: V Lorenzo-Zúñiga; C M Rodríguez-Ortigosa; R Bartolí; M-L Martínez-Chantar; L Martínez-Peralta; A Pardo; I Ojanguren; J Quiroga; R Planas; J Prieto Journal: Gut Date: 2006-01-24 Impact factor: 23.059
Authors: A Cemborain; I Castilla-Cortázar; M García; B Muguerza; G Delgado; M Díaz-Sánchez; A Picardi Journal: J Physiol Biochem Date: 2000-06 Impact factor: 4.158
Authors: I Castilla-Cortazar; L Guerra; J E Puche; U Muñoz; R Barhoum; E Escudero; J L Lavandera Journal: J Physiol Biochem Date: 2013-09-18 Impact factor: 4.158
Authors: Gemma Odena; Mireia Miquel; Anna Serafín; Amparo Galan; Rosa Morillas; Ramon Planas; Ramon Bartolí Journal: World J Gastroenterol Date: 2012-05-07 Impact factor: 5.742