Literature DB >> 9322302

An experimental study on application of PCR in detection of kala-azar.

Z Wu1, Y Bao, Y Ding, M Yu, L Lu, Y Zhang.   

Abstract

A polymerase chain reaction (PCR) technique was developed to detect Kala-azar. Out of 25 patients, 24 were detected, a sensitivity of 96%. The specificity rate, false positive rate, false negative rate, coincidence rate and Youdent's index were 100% (60/60), 0, 4%, 99% and 0.96 respectively. It was shown that the PCR technique can directly detect Kala-azar in samples (1 microliter) from peripheral blood.

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Year:  1997        PMID: 9322302

Source DB:  PubMed          Journal:  Southeast Asian J Trop Med Public Health        ISSN: 0125-1562            Impact factor:   0.267


  5 in total

Review 1.  Molecular Diagnosis of Visceral Leishmaniasis.

Authors:  Shyam Sundar; Om Prakash Singh
Journal:  Mol Diagn Ther       Date:  2018-08       Impact factor: 4.074

2.  Detection of Leishmania infantum in dogs by PCR with lymph node aspirates and blood.

Authors:  S Reale; L Maxia; F Vitale; N S Glorioso; S Caracappa; G Vesco
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

Review 3.  Molecular tools for diagnosis of visceral leishmaniasis: systematic review and meta-analysis of diagnostic test accuracy.

Authors:  C M de Ruiter; C van der Veer; M M G Leeflang; S Deborggraeve; C Lucas; E R Adams
Journal:  J Clin Microbiol       Date:  2014-05-14       Impact factor: 5.948

Review 4.  A critical review of the applicability of serological screening for Leishmaniasis in blood banks in Brazil.

Authors:  Wellington Francisco Rodrigues; Niege Silva Mendes; Patrícia de Carvalho Ribeiro; Daniel Mendes Filho; Ricardo Cambraia Parreira; Karen Cristina Barbosa Chaves; Melissa Carvalho Martins de Abreu; Camila Botelho Miguel
Journal:  J Parasit Dis       Date:  2020-10-08

5.  Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum.

Authors:  Fernanda Alvarenga Cardoso Medeiros; Luciana Inácia Gomes; Edward Oliveira; Carolina Senra Alves de Souza; Maria Vitória Mourão; Gláucia Fernandes Cota; Letícia Helena Dos Santos Marques; Mariângela Carneiro; Ana Rabello
Journal:  J Trop Med       Date:  2017-01-09
  5 in total

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