BACKGROUND: Olive tree (Olea europaea) pollen allergy is one of the main causes of allergy in Mediterranean countries and some areas of North America. OBJECTIVE: To clone olive allergens and to characterize immunologically the purified recombinant allergens. METHODS: Full-length complementary deoxyribonucleic acid (cDNA) strands encoding olive allergens (Ole e 1) were cloned by polymerase chain reaction amplification and sequenced. Recombinant proteins were produced in Escherichia coli by the use of two different expression systems. Immunoreactivity of the recombinant proteins was tested by ELISA and Western blot with serum from patients with allergy to olive. RESULTS: Significant sequence polymorphism was found in both allergens. The panallergen profilin was expressed as a nonfusion protein and was purified to homogeneity after a single step of affinity chromatography with a poly-L-proline Sepharose column. One cDNA encoding an Ole e 1 isoform was expressed as a fusion protein consisting of the glutathione S-transferase of Schistosoma japonicum and Ole e 1. The fusion protein was purified to homogeneity by gel filtration chromatography and affinity chromatography with a glutathione-Sepharose column, and digested with thrombin. Both recombinant allergens shared B cell epitopes with the corresponding natural allergens. CONCLUSION: IgE-reactive Ole e 1 and olive profilin expressed in bacteria were purified after simple chromatographic procedures and may be useful for diagnostic purposes.
BACKGROUND:Olive tree (Olea europaea) pollen allergy is one of the main causes of allergy in Mediterranean countries and some areas of North America. OBJECTIVE: To clone olive allergens and to characterize immunologically the purified recombinant allergens. METHODS: Full-length complementary deoxyribonucleic acid (cDNA) strands encoding olive allergens (Ole e 1) were cloned by polymerase chain reaction amplification and sequenced. Recombinant proteins were produced in Escherichia coli by the use of two different expression systems. Immunoreactivity of the recombinant proteins was tested by ELISA and Western blot with serum from patients with allergy to olive. RESULTS: Significant sequence polymorphism was found in both allergens. The panallergen profilin was expressed as a nonfusion protein and was purified to homogeneity after a single step of affinity chromatography with a poly-L-proline Sepharose column. One cDNA encoding an Ole e 1 isoform was expressed as a fusion protein consisting of the glutathione S-transferase of Schistosoma japonicum and Ole e 1. The fusion protein was purified to homogeneity by gel filtration chromatography and affinity chromatography with a glutathione-Sepharose column, and digested with thrombin. Both recombinant allergens shared B cell epitopes with the corresponding natural allergens. CONCLUSION: IgE-reactive Ole e 1 and olive profilin expressed in bacteria were purified after simple chromatographic procedures and may be useful for diagnostic purposes.
Authors: Abdol-Reza Varasteh; Mojtaba Sankian; Terumi Midoro-Horiuti; Malihe Moghadam; Mohamad Taghi Shakeri; Edward G Brooks; Randall M Goldblum; Martin D Chapman; Anna Pomés Journal: Rep Biochem Mol Biol Date: 2012-10
Authors: Jose C Jimenez-Lopez; Sonia Morales; Antonio J Castro; Dieter Volkmann; María I Rodríguez-García; Juan de D Alché Journal: PLoS One Date: 2012-02-14 Impact factor: 3.240
Authors: Mohamed Elfatih H Bashir; Jason M Ward; Matthew Cummings; Eltayeb E Karrar; Michael Root; Abu Bekr A Mohamed; Robert M Naclerio; Daphne Preuss Journal: PLoS One Date: 2013-01-07 Impact factor: 3.240