DNA sequence analysis of an avian adenovirus terminal protein precursor.

Nucleotide sequence of the genomic region between map units 25 and 31 of the fowl adenovirus serotype 10 (FAV 10) was determined and analyzed. An open reading frame (ORF) running from right to left (that is on /-strand) of 1806 nucleotides in length was found. This ORF encoded a polypeptide of 602 amino acids with a molecular weight (M[r]) of approximately 70.4 kilo-Daltons. The genomic location of the ORF was determined to be between map units 25.5 and 29.5, similar to the genomic position of the human adenovirus (HAV) terminal protein precursor (pTP). From its size, approximate genomic location and direction of transcription, this ORF was suspected to be the FAV10 homologue of the pTP. Amino acid sequence comparison with the HAV2 pTP revealed an amino acid sequence similarity of 32.4% but was 51 amino acids shorter in length. A potential proteolytic cleavage site was identified which would create a post-cleavage terminal protein of 316 amino acids, again comparable to the 322 amino acids of the post-cleavage TP of HAV.