Literature DB >> 9306659

Rapid identification of Clostridium botulinum and botulinal toxin in food.

A Rodriguez1, M Dezfulian.   

Abstract

Samples of green beans and mushrooms were inoculated with a toxigenic strain of Clostridium botulinum type A and incubated anaerobically at 37 degrees C. At various time intervals, the seeded food samples were tested for the presence of botulinal toxin and C. botulinum by an agar plating method and an enzyme-linked immunosorbent assay. C. botulinum type A that appeared as lipase-positive colonies on selective agar plates, and its elaborated toxin, were identified in all seeded food samples within 1 to 2 d. This procedure can be adapted for rapid screening of suspected food samples.

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Year:  1997        PMID： 9306659     DOI： 10.1007/BF02898726

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.629


  7 in total

1.  Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.

Authors:  M Dezfulian; J G Bartlett
Journal:  J Clin Microbiol       Date:  1984-05       Impact factor: 5.948

2.  Laboratory diagnosis of botulism complicated by pyridostigmine treatment of the patient. A method for selectively removing interfering substances from clinical specimens.

Authors:  M A Horwitz; C L Hatheway; V R Dowell
Journal:  Am J Clin Pathol       Date:  1976-10       Impact factor: 2.493

3.  Coproexamination for botulinal toxin and clostridium botulinum. A new procedure for laboratory diagnosis of botulism.

Authors:  V R Dowell; L M McCroskey; C L Hatheway; G L Lombard; J M Hughes; M H Merson
Journal:  JAMA       Date:  1977-10-24       Impact factor: 56.272

4.  Selective medium for isolation of Clostridium botulinum from human feces.

Authors:  M Dezfulian; L M McCroskey; C L Hatheway; V R Dowell
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

5.  Detection of Clostridium botulinum type B toxin in the presence of a lethal substance interfering with toxin neutralization.

Authors:  M Dezfulian; J G Bartlett
Journal:  Diagn Microbiol Infect Dis       Date:  1985-03       Impact factor: 2.803

6.  Selective isolation and rapid identification of Clostridium botulinum types A and B by toxin detection.

Authors:  M Dezfulian; J G Bartlett
Journal:  J Clin Microbiol       Date:  1985-02       Impact factor: 5.948

7.  Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type A and type B toxins in stool samples of infants with botulism.

Authors:  M Dezfulian; C L Hatheway; R H Yolken; J G Bartlett
Journal:  J Clin Microbiol       Date:  1984-09       Impact factor: 5.948
  7 in total
  4 in total

Review 1.  Laboratory diagnostics of botulism.

Authors:  Miia Lindström; Hannu Korkeala
Journal:  Clin Microbiol Rev       Date:  2006-04       Impact factor: 26.132

2.  Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies.

Authors:  Shashi K Sharma; Joseph L Ferreira; Brian S Eblen; Richard C Whiting
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792

3.  Universal and specific quantitative detection of botulinum neurotoxin genes.

Authors:  Brenna J Hill; Janet C Skerry; Theresa J Smith; Stephen S Arnon; Daniel C Douek
Journal:  BMC Microbiol       Date:  2010-10-20       Impact factor: 3.605

4.  Sensing the deadliest toxin: technologies for botulinum neurotoxin detection.

Authors:  Petr Capek; Tobin J Dickerson
Journal:  Toxins (Basel)       Date:  2010-01-07       Impact factor: 4.546
  4 in total

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