| Literature DB >> 9305844 |
D Kim1, K S Jun, S B Lee, N G Kang, D S Min, Y H Kim, S H Ryu, P G Suh, H S Shin.
Abstract
A variety of extracellular signals are transduced across the cell membrane by the enzyme phosphoinositide-specific phospholipase C-beta (PLC-beta) coupled with guanine-nucleotide-binding G proteins. There are four isoenzymes of PLC-beta, beta1-beta4, but their functions in vivo are not known. Here we investigate the role of PLC-beta1 and PLC-beta4 in the brain by generating null mutations in mice: we found that PLCbeta1-/- mice developed epilepsy and PLCbeta4-/- mice showed ataxia. We determined the molecular basis of these phenotypes and show that PLC-beta1 is involved in signal transduction in the cerebral cortex and hippocampus by coupling predominantly to the muscarinic acetylcholine receptor, whereas PLC-beta4 works through the metabotropic glutamate receptor in the cerebellum, illustrating how PLC-beta isoenzymes are used to generate different functions in the brain.Entities:
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Year: 1997 PMID： 9305844 DOI： 10.1038/38508
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962