Literature DB >> 9302651

Characterization of microsomal GST-II by western blot and identification of a novel LTC4 isomer.

P J Jakobsson1, K A Scoggan, J Yergey, J A Mancini, A W Ford-Hutchinson.   

Abstract

Protein expression of microsomal GST-II and LTC4 synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC4 formation was observed for both enzymes. The expression of microsomal GST-II was several fold more efficient than the expression of LTC4 synthase. In addition to catalyzing the biosynthesis of LTC4, microsomal GST-II also produces another product, which has been subjected to mass spectrometric analysis. This analysis demonstrates that the novel product is an isomer of LTC4.

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Year:  1997        PMID: 9302651     DOI: 10.1016/s0929-7855(97)00013-8

Source DB:  PubMed          Journal:  J Lipid Mediat Cell Signal        ISSN: 0929-7855


  2 in total

1.  Arginine 104 is a key catalytic residue in leukotriene C4 synthase.

Authors:  Agnes Rinaldo-Matthis; Anders Wetterholm; Daniel Martinez Molina; Johanna Holm; Damian Niegowski; Eva Ohlson; Pär Nordlund; Ralf Morgenstern; Jesper Z Haeggström
Journal:  J Biol Chem       Date:  2010-10-27       Impact factor: 5.157

2.  Developmental changes in the transcriptome of the rat choroid plexus in relation to neuroprotection.

Authors:  Ingrid Kratzer; Shane A Liddelow; Norman R Saunders; Kate M Dziegielewska; Nathalie Strazielle; Jean-Francois Ghersi-Egea
Journal:  Fluids Barriers CNS       Date:  2013-08-01
  2 in total

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