Literature DB >> 9302218

Dilution assessment of cervicovaginal secretions collected by vaginal washing to evaluate mucosal shedding of free human immunodeficiency virus.

A S Mohamed1, P Becquart, H Hocini, P Métais, M Kazatchkine, L Bélec.   

Abstract

A 10 mM concentration of lithium does not interfere with reverse transcription (RT) or PCR. Sampling of cervicovaginal fluid by vaginal washing, with lithium (10 mM) in the washing buffer as a marker of dilution, may be utilized to accurately determine in HIV-infected women, by quantitative RT-PCR, the genital shedding of acellular HIV RNA at the level of the mucosa itself.

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Year:  1997        PMID： 9302218      PMCID： PMC170612          DOI： 10.1128/cdli.4.5.624-626.1997

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  6 in total

1.  Presence of cell-free human immunodeficiency virus in cervicovaginal secretions is independent of viral load in the blood of human immunodeficiency virus-infected women.

Authors:  S Rasheed; Z Li; D Xu; A Kovacs
Journal:  Am J Obstet Gynecol       Date:  1996-07       Impact factor: 8.661

Review 2.  Shedding of HIV-1 in the genital tract.

Authors:  S B Mostad; J K Kreiss
Journal:  AIDS       Date:  1996-10       Impact factor: 4.177

3.  A longitudinal study of human immunodeficiency virus transmission by heterosexual partners. European Study Group on Heterosexual Transmission of HIV.

Authors:  I de Vincenzi
Journal:  N Engl J Med       Date:  1994-08-11       Impact factor: 91.245

4.  Detection of HIV DNA in cervical and vaginal secretions. Prevalence and correlates among women in Nairobi, Kenya.

Authors:  D B Clemetson; G B Moss; D M Willerford; M Hensel; W Emonyi; K K Holmes; F Plummer; J Ndinya-Achola; P L Roberts; S Hillier
Journal:  JAMA       Date:  1993-06-09       Impact factor: 56.272

5.  Dilution assessment of cervicovaginal secretions obtained by vaginal washing for immunological assays.

Authors:  L Bélec; D Meillet; M Lévy; A Georges; C Tévi-Bénissan; J Pillot
Journal:  Clin Diagn Lab Immunol       Date:  1995-01

6.  Standardisation of primers and an algorithm for HIV-1 diagnostic PCR evaluated in patients harbouring strains of diverse geographical origin. The Belgian AIDS Reference Laboratories.

Authors:  A M Vandamme; K Fransen; L Debaisieux; D Marissens; S Sprecher; D Vaira; A T Vandenbroucke; C Verhofstede
Journal:  J Virol Methods       Date:  1995-02       Impact factor: 2.014
  6 in total
  3 in total

1.  Cervicovaginal shedding of HIV type 1 is related to genital tract inflammation independent of changes in vaginal microbiota.

Authors:  Caroline Mitchell; Jane Hitti; Kathleen Paul; Kathy Agnew; Susan E Cohn; Amneris E Luque; Robert Coombs
Journal:  AIDS Res Hum Retroviruses       Date:  2010-10-07       Impact factor: 2.205

2.  Characterization of HIV-1 gp120 antibody specificities induced in anogenital secretions of RV144 vaccine recipients after late boost immunizations.

Authors:  Siriwat Akapirat; Chitraporn Karnasuta; Sandhya Vasan; Supachai Rerks-Ngarm; Punnee Pitisuttithum; Sirinan Madnote; Hathairat Savadsuk; Surawach Rittiroongrad; Jiraporn Puangkaew; Sanjay Phogat; James Tartaglia; Faruk Sinangil; Mark S de Souza; Jean-Louis Excler; Jerome H Kim; Merlin L Robb; Nelson L Michael; Viseth Ngauy; Robert J O'Connell; Nicos Karasavvas
Journal:  PLoS One       Date:  2018-04-27       Impact factor: 3.240

3.  MTN-001: randomized pharmacokinetic cross-over study comparing tenofovir vaginal gel and oral tablets in vaginal tissue and other compartments.

Authors:  Craig W Hendrix; Beatrice A Chen; Vijayanand Guddera; Craig Hoesley; Jessica Justman; Clemensia Nakabiito; Robert Salata; Lydia Soto-Torres; Karen Patterson; Alexandra M Minnis; Sharavi Gandham; Kailazarid Gomez; Barbra A Richardson; Namandje N Bumpus
Journal:  PLoS One       Date:  2013-01-30       Impact factor: 3.240
  3 in total

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