Literature DB >> 9301988

Effects of biocides on Acanthamoeba castellanii as measured by flow cytometry and plaque assay.

W Khunkitti1, S V Avery, D Lloyd, J R Furr, A D Russell.   

Abstract

The lethal effects of various biocides, in particular two agents (chlorhexidine diacetate and polyhexamethylene biguanide) on cysts and trophozoites of Acanthamoeba castellanii have been measured by both plaque assay and flow cytometry (FCM). Minimum amoebicidal concentrations against trophozoites were generally the same by the two methods whereas minimum cysticidal concentrations were occasionally higher by FCM. Concentrations of biguanides required to produce an equal reduction in viability were also higher by FCM than by plaque assay.

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Year:  1997        PMID: 9301988     DOI: 10.1093/jac/40.2.227

Source DB:  PubMed          Journal:  J Antimicrob Chemother        ISSN: 0305-7453            Impact factor:   5.790


  8 in total

1.  Flow cytometry for determination of the efficacy of contact lens disinfecting solutions against Acanthamoeba spp.

Authors:  R N Borazjani; L L May; J A Noble; S V Avery; D G Ahearn
Journal:  Appl Environ Microbiol       Date:  2000-03       Impact factor: 4.792

2.  In vitro comparative assessment of different viability assays in Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites.

Authors:  I Heredero-Bermejo; J L Copa-Patiño; J Soliveri; R Gómez; F J de la Mata; J Pérez-Serrano
Journal:  Parasitol Res       Date:  2013-09-12       Impact factor: 2.289

3.  Use of 5-cyano-2,3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents.

Authors:  Takeshi Kobayashi; Tsuyoshi Mito; Narumi Watanabe; Takashi Suzuki; Atsushi Shiraishi; Yuichi Ohashi
Journal:  J Clin Microbiol       Date:  2012-02-15       Impact factor: 5.948

4.  Evaluations of shorter exposures of contact lens cleaning solutions against Fusarium oxysporum species complex and Fusarium solani species complex to simulate inappropriate usage.

Authors:  Rama Ramani; Vishnu Chaturvedi
Journal:  Antimicrob Agents Chemother       Date:  2011-02-07       Impact factor: 5.191

5.  Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba.

Authors:  James McBride; Paul R Ingram; Fiona L Henriquez; Craig W Roberts
Journal:  J Clin Microbiol       Date:  2005-02       Impact factor: 5.948

6.  Oxygen induction of a novel fatty acid n-6 desaturase in the soil protozoon, Acanthamoeba castellanii.

Authors:  Andrew J Rutter; Katie L Thomas; Derek Herbert; R James Henderson; David Lloyd; John L Harwood
Journal:  Biochem J       Date:  2002-11-15       Impact factor: 3.857

Review 7.  Antiseptics and disinfectants: activity, action, and resistance.

Authors:  G McDonnell; A D Russell
Journal:  Clin Microbiol Rev       Date:  1999-01       Impact factor: 26.132

8.  Nisin Induces Cell-Cycle Arrest in Free-Living Amoebae Acanthamoeba castellanii.

Authors:  Marianna de Carvalho Clímaco; Yrna Lorena Matos de Oliveira; Anne Caroline Santos Ramos; Jucicleide Ramos-de-Souza; Audrey Rouse Soares Tavares Silva; Sona Jain; Marilise Brittes Rott; Ricardo Scher; Cristiane Bani Correa; Ana Andrea Teixeira Barbosa; Silvio Santana Dolabella
Journal:  Acta Parasitol       Date:  2021-06-22       Impact factor: 1.440

  8 in total

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