Literature DB >> 9299470

A novel aortic smooth muscle cell line obtained from p53 knock out mice expresses several differentiation characteristics.

K Ohmi1, T Masuda, H Yamaguchi, T Sakurai, Y Kudo, M Katsuki, Y Nonomura.   

Abstract

Here we report that we could obtain a highly differentiated smooth muscle cell line by screening the expression of a-smooth muscle actin from p53 knook out mice aorta. This cell revealed extended bipolar shape and expressed h-caldesmon and calponin as well as a-smooth muscle actin as protein markers of differentiated smooth muscle. Further intracellular calcium increase was induced by application of noradrenaline in a dose dependent manner and calcium oscillation was also observed in a higher dose (100 microM). Appropriate application of 5-azacytidine enhanced these tendencies and induced slow contraction by endothelin-1 and phenylephrine.

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Year:  1997        PMID： 9299470     DOI： 10.1006/bbrc.1997.7218

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Novel transcripts of Nox1 are regulated by alternative promoters and expressed under phenotypic modulation of vascular smooth muscle cells.

Authors:  Noriaki Arakawa; Masato Katsuyama; Kuniharu Matsuno; Norifumi Urao; Yoshiaki Tabuchi; Mitsuhiko Okigaki; Hiroaki Matsubara; Chihiro Yabe-Nishimura
Journal:  Biochem J       Date:  2006-09-01       Impact factor: 3.857

2.  Growing and differentiating characterization of aortic smooth muscle cell line, p53LMAC01 obtained from p53 knock out mice.

Authors:  T Masuda; K Ohmi; H Yamaguchi; K Hasegawa; T Sugiyama; Y Matsuda; M Iino; Y Nonomura
Journal:  Mol Cell Biochem       Date:  1999-01       Impact factor: 3.396

3.  Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity via Nox1-dependent auto-dephosphorylation of serine 834 in vascular smooth muscle cells.

Authors:  Mithu Maheswaranathan; Hope K A Gole; Isabel Fernandez; Bernard Lassègue; Kathy K Griendling; Alejandra San Martín
Journal:  J Biol Chem       Date:  2011-08-20       Impact factor: 5.157

4.  Characterization of alpha 1-adrenoceptors expressed in a novel vascular smooth muscle cell line cloned from p53 knockout mice, P53LMAC01 (AC01) cells.

Authors:  K Ohmi; H Shinoura; Y Nakayama; N Goda; G Tsujimoto
Journal:  Br J Pharmacol       Date:  1999-06       Impact factor: 8.739

5.  miR181a protects against angiotensin II-induced osteopontin expression in vascular smooth muscle cells.

Authors:  Ebony Washington Remus; Alicia N Lyle; Daiana Weiss; Natalia Landàzuri; Martina Weber; Charles Searles; W Robert Taylor
Journal:  Atherosclerosis       Date:  2013-02-05       Impact factor: 5.162

6.  Mechanisms of vascular smooth muscle NADPH oxidase 1 (Nox1) contribution to injury-induced neointimal formation.

Authors:  Moo Yeol Lee; Alejandra San Martin; Puja K Mehta; Anna E Dikalova; Abel Martin Garrido; S Raju Datla; Erin Lyons; Karl-Heinz Krause; Botond Banfi; J David Lambeth; Bernard Lassègue; Kathy K Griendling
Journal:  Arterioscler Thromb Vasc Biol       Date:  2009-01-15       Impact factor: 8.311

7.  8-Hydroxy-2-deoxyguanosine prevents plaque formation and inhibits vascular smooth muscle cell activation through Rac1 inactivation.

Authors:  Joo Young Huh; Dong Ju Son; Yoonji Lee; Junghyun Lee; Boyeon Kim; Hwan Myung Lee; Hanjoong Jo; Sun Choi; Hunjoo Ha; Myung-Hee Chung
Journal:  Free Radic Biol Med       Date:  2012-04-19       Impact factor: 7.376

8.  Assembly of smooth muscle myosin by the 38k protein, a homologue of a subunit of pre-mRNA splicing factor-2.

Authors:  T Okagaki; A Nakamura; T Suzuki; K Ohmi; K Kohama
Journal:  J Cell Biol       Date:  2000-02-21       Impact factor: 10.539

9.  p53 negatively regulates the osteogenic differentiation of vascular smooth muscle cells in mice with chronic kidney disease.

Authors:  K L Li; J Chen; Z H Li; L Zhao; Y N He
Journal:  Cardiovasc J Afr       Date:  2012-07-12       Impact factor: 1.167
  9 in total

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