Literature DB >> 9299462

Primer/template-independent synthesis of poly d(A-T) by Taq polymerase.

K Hanaki1, T Odawara, T Muramatsu, Y Kuchino, M Masuda, K Yamamoto, C Nozaki, K Mizuno, H Yoshikura.   

Abstract

Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.

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Year:  1997        PMID： 9299462     DOI： 10.1006/bbrc.1997.7197

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

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Review 8.  It Is Imperative to Establish a Pellucid Definition of Chimeric RNA and to Clear Up a Lot of Confusion in the Relevant Research.

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