| Literature DB >> 9293080 |
N C Leal1, F G Abath, L C Alves, A M de Almeida.
Abstract
Supernatant of boiled spleen saline-suspensions of Yersinia pestis experimentally infected animals were used as template for PCR amplification without DNA extraction. PCR sensitivity was enhanced by a second round of amplification (Nested). No amplification was observed from non-infected animals.Entities:
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Year: 1996 PMID： 9293080 DOI： 10.1590/s0036-46651996000500009
Source DB: PubMed Journal: Rev Inst Med Trop Sao Paulo ISSN： 0036-4665 Impact factor: 1.846