Gene expression of catecholamine synthesizing enzymes and beta adrenoceptor subtypes during rat embryogenesis.

Timed-pregnant Sprague-Dawley rats were killed between gestational day (GD) 8 and 10, and embryos were explanted and separated into developmental stages according to a modified Theiler's system. Total RNA from each stage was isolated and subjected to reverse transcription-polymerase chain reaction (RT-PCR) assays to examine gene expression of catecholamine synthesizing enzymes and three subtypes of beta adrenoceptors. Expression of these genes was detected at much earlier stages than previously reported, and each enzyme and receptor subtype showed a different pattern of gene expression. For example, mRNA for tyrosine hydroxylase, the rate-limiting enzyme for catecholamine synthesis, was detected as early as stage 10a, late GD 8, before the neural crest cells appear (stage 12, mid GD 10). This contradicts the common belief that catecholamines are produced only in the cells of sympathoadrenal lineage which originate from the neural crest cells and the cells of central nervous system. Results from the present study indicate that catecholamine synthesis is not limited to the cells of sympathoadrenal lineage.