BACKGROUND: In islet transplantation pancreatic preservation before islet isolation is an obstacle compromising islet yield and viability. We tested the feasibility of a two-layer method (University of Wisconsin solution [UW]/perfluorochemical) for pancreatic preservation before islet isolation. METHODS: Dog pancreases were processed into pure islets by the method of Ricordi preceded by five different preservations (groups 1-a and 1-b, the two-layer method for 3 and 24 hours; groups 2-a and 2-b, simple cold storage in UW for 3 and 24 hours; group 3, without preservation). Islet yields and functional success after autotransplantation into the liver were compared among the groups. RESULTS: Postpurification islet equivalents (IE)/gm pancreas and functional success rate were 5600 (mean), 83% in group 1-a; 4000, 56% in group 1-b; 4700, 33% in group 2-a; 1300, 0% in group 2-b; and 5000, 89% in group 3 (p < 0.05; 2b versus 1-a, 1-b, and 3), respectively. There was no statistical difference among groups 1-a, 1-b, and 3 in terms of islet yield and function (p > 0.2). CONCLUSIONS: The two-layer method is more effective than conventional simple cold storage in UW for pancreatic preservation before islet isolation. Clinical trials with the two-layer method are warranted.
BACKGROUND: In islet transplantation pancreatic preservation before islet isolation is an obstacle compromising islet yield and viability. We tested the feasibility of a two-layer method (University of Wisconsin solution [UW]/perfluorochemical) for pancreatic preservation before islet isolation. METHODS:Dog pancreases were processed into pure islets by the method of Ricordi preceded by five different preservations (groups 1-a and 1-b, the two-layer method for 3 and 24 hours; groups 2-a and 2-b, simple cold storage in UW for 3 and 24 hours; group 3, without preservation). Islet yields and functional success after autotransplantation into the liver were compared among the groups. RESULTS: Postpurification islet equivalents (IE)/gm pancreas and functional success rate were 5600 (mean), 83% in group 1-a; 4000, 56% in group 1-b; 4700, 33% in group 2-a; 1300, 0% in group 2-b; and 5000, 89% in group 3 (p < 0.05; 2b versus 1-a, 1-b, and 3), respectively. There was no statistical difference among groups 1-a, 1-b, and 3 in terms of islet yield and function (p > 0.2). CONCLUSIONS: The two-layer method is more effective than conventional simple cold storage in UW for pancreatic preservation before islet isolation. Clinical trials with the two-layer method are warranted.
Authors: S A White; J E Davies; C Pollard; S M Swift; H A Clayton; C D Sutton; S Weymss-Holden; P P Musto; D P Berry; A R Dennison Journal: Ann Surg Date: 2001-03 Impact factor: 12.969
Authors: A N Balamurugan; B Naziruddin; A Lockridge; M Tiwari; G Loganathan; M Takita; S Matsumoto; K Papas; M Trieger; H Rainis; T Kin; T W Kay; S Wease; S Messinger; C Ricordi; R Alejandro; J Markmann; J Kerr-Conti; M R Rickels; C Liu; X Zhang; P Witkowski; A Posselt; P Maffi; A Secchi; T Berney; P J O'Connell; B J Hering; F B Barton Journal: Am J Transplant Date: 2014-10-02 Impact factor: 8.086
Authors: Horacio L Rodriguez Rilo; Syed A Ahmad; David D'Alessio; Yasuhiro Iwanaga; Joseph Kim; Kyuran A Choe; Jonathan S Moulton; Jill Martin; Linda J Pennington; Debbie A Soldano; Jamie Biliter; Steve P Martin; Charles D Ulrich; Lehel Somogyi; Jeffrey Welge; Jeffrey B Matthews; Andrew M Lowy Journal: J Gastrointest Surg Date: 2003-12 Impact factor: 3.267