Effect of manipulation of the renin-angiotensin system in control of drinking in juvenile Atlantic salmon (Salmo salar L) in fresh water and after transfer to sea water.

Drinking in Atlantic salmon (Salmo salar) juveniles was investigated in fresh water and following transfer to sea water. There was a significant effect of fish size on drinking, and smolts (20-30 g) imbibed about ten times less water than alevins of 0.2-0.3 g. Freshwater smolts drank at a rate of 0.15 +/- 0.03 ml.kg-1.h-1 and administration of doses of 10 or 20 mg.kg-1 of papaverine (stimulator of the renin-angiotensin system RAS) or [Asn1, Val5]-Angiotensin II (0.4 mumol.kg-1) resulted in significant increases in drinking, while administration of the angiotensin converting enzyme inhibitor, enalapril (50 mg.kg-1) had no effect on drinking. Transfer of Atlantic salmon smolts to 1/3, 2/3 and full strength sea water resulted in significant increases in drinking to 1.06 +/- 0.12, 1.24 +/- 0.0.16 and 3.89 +/- 0.28 ml.kg-1.h-1, respectively. In sea water, stimulation of the endogenous RAS by administration of papaverine (20 mg.kg-1) resulted in a 20% increase in drinking, while administration of enalapril to doses of 50 and 200 mg.kg-1 lowered drinking to 1.99 +/- 0.48 and 0.32 +/- 0.06 ml.kg-1.h-1, respectively. All treatments were without effect on blood plasma levels of Na+ and Cl- in fresh water, while in sea water smolts both stimulation and inhibition of drinking resulted in hemoconcentration of Na+ and Cl-. The role of the renin angiotensin system in control of drinking and hydromineral balance in Atlantic salmon is discussed.