Literature DB >> 9281411

PCR detection of Ruminococcus spp. in human and animal faecal samples.

R F Wang1, W W Cao, C E Cerniglia.   

Abstract

Polymerase chain reaction (PCR) procedures for Ruminococcus spp. were developed and used for the detection of Ruminococcus spp. in human and animal faeces. The PCR specificity was established for Ruminococcus albus, R. bromii, R. obeum, R. callidus and R. flavefaciens. The PCR sensitivity ranged from 4 to 100 cells of the pure cultures, depending on the species. Ruminococcus albus, R. bromii, R. obeum and R. callidus were detected in human faeces. R. bromii, R. albus and R. obeum were also detected in animal faeces. R. flavefaciens was not detected in most of the samples tested, except for a weak positive in monkey faecal specimens. The PCR appears to be a specific and efficient method for the detection of Ruminococcus spp. in faecal specimens.

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Year:  1997        PMID： 9281411     DOI： 10.1006/mcpr.1997.0111

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  18 in total

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