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Literature DB >> 9278502

Multiplex genotyping of PCR products with MassTag-labeled primers.

Abstract

A simple mass spectrometric based assay, the PinPoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.

Mesh：

Substances：
DNA Primers

Year: 1997 PMID： 9278502 PMCID： PMC146949 DOI： 10.1093/nar/25.18.3749

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

3 in total

1. Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry.

Authors: L A Haff; I P Smirnov
Journal: Genome Res Date: 1997-04 Impact factor: 9.043

2. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1.

Authors: Y Miki; J Swensen; D Shattuck-Eidens; P A Futreal; K Harshman; S Tavtigian; Q Liu; C Cochran; L M Bennett; W Ding
Journal: Science Date: 1994-10-07 Impact factor: 47.728

3. Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms.

Authors: T T Nikiforov; R B Rendle; P Goelet; Y H Rogers; M L Kotewicz; S Anderson; G L Trainor; M R Knapp
Journal: Nucleic Acids Res Date: 1994-10-11 Impact factor: 16.971

3 in total

19 in total

1. A new MALDI-TOF based mini-sequencing assay for genotyping of SNPS.

Authors: X Sun; H Ding; K Hung; B Guo
Journal: Nucleic Acids Res Date: 2000-06-15 Impact factor: 16.971

2. Rapid characterization of DNA oligomers and genotyping of single nucleotide polymorphism using nucleotide-specific mass tags.

Authors: F Abdi; E M Bradbury; N Doggett; X Chen
Journal: Nucleic Acids Res Date: 2001-07-01 Impact factor: 16.971

Multiplex genotyping of PCR products with MassTag-labeled primers.

1. Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry.

2. A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1.

3. Genetic Bit Analysis: a solid phase method for typing single nucleotide polymorphisms.

1. A new MALDI-TOF based mini-sequencing assay for genotyping of SNPS.

2. Rapid characterization of DNA oligomers and genotyping of single nucleotide polymorphism using nucleotide-specific mass tags.

3. Hierarchical high-throughput SNP genotyping of the human Y chromosome using MALDI-TOF mass spectrometry.

4. Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.

5. MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging.

6. A FRET-based analysis of SNPs without fluorescent probes.

Review 7. Forensically relevant SNaPshot^® assays for human DNA SNP analysis: a review.

8. MALDI-TOF mass spectrometric typing of single nucleotide polymorphisms with mass-tagged ddNTPs.

9. Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry.

10. Function of the HVCN1 proton channel in airway epithelia and a naturally occurring mutation, M91T.

Review 7. Forensically relevant SNaPshot® assays for human DNA SNP analysis: a review.

Review 7. Forensically relevant SNaPshot^® assays for human DNA SNP analysis: a review.