Literature DB >> 9278489

A new method of synthesis of fluorescently labelled oligonucleotides and their application in DNA sequencing.

W T Markiewicz1, G Gröger, R Rösch, A Zebrowska, M Markiewicz, M Klotz, M Hinz, P Godzina, H Seliger.   

Abstract

A new approach to the chemical synthesis of oligodeoxynucleotides bearing reporter functional groups at base residues of 3'-end nucleosides is reported. Applications of the 3'-end fluorescently labelled primers for automated DNA sequencing are shown.

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Year:  1997        PMID： 9278489      PMCID： PMC146946          DOI： 10.1093/nar/25.18.3672

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

Review 1.  Cloning in M13 phage or how to use biology at its best.

Authors:  J Messing
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

2.  Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin).

Authors:  K Mühlegger; E Huber; H von der Eltz; R Rüger; C Kessler
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

3.  Automated sequencing of fluorescently labelled DNA by chemical degradation.

Authors:  A Rosenthal; B Sproat; H Voss; J Stegemann; C Schwager; H Erfle; J Zimmermann; C Coutelle; W Ansorge
Journal:  DNA Seq       Date:  1990

4.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

5.  Immunocytochemistry of 5-bromo-2'-deoxyuridine labelled oligonucleotide probes. A novel technique for in situ hybridization.

Authors:  G F Jirikowski; J F Ramalho-Ortigao; T Lindl; H Seliger
Journal:  Histochemistry       Date:  1989

6.  Preparation and chromatographic use of 5'-fluorescent-labelled DNA probes.

Authors:  G Tous; J Fausnaugh; P Vieira; S Stein
Journal:  J Chromatogr       Date:  1988-07-01

7.  Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides.

Authors:  K Misiura; I Durrant; M R Evans; M J Gait
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

8.  A protected biotin containing deoxycytidine building block for solid phase synthesis of biotinylated oligonucleotides.

Authors:  U Pieles; B S Sproat; G M Lamm
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

9.  Guanine modification during chemical DNA synthesis.

Authors:  J S Eadie; D S Davidson
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

10.  Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis.

Authors:  H R Starke; J Y Yan; J Z Zhang; K Mühlegger; K Effgen; N J Dovichi
Journal:  Nucleic Acids Res       Date:  1994-09-25       Impact factor: 16.971
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  2 in total

1.  Synthesis and fluorescence studies of some new fluorophores and their effect on hybridization of oligodeoxyribonucleotides.

Authors:  Shipra Singh; Ramendra K Singh
Journal:  J Fluoresc       Date:  2007-01-18       Impact factor: 2.217

2.  Synthesis and evaluation of phosphorescent oligonucleotide probes for hybridisation assays.

Authors:  Paul J O'Sullivan; Martina Burke; Aleksi E Soini; Dmitri B Papkovsky
Journal:  Nucleic Acids Res       Date:  2002-11-01       Impact factor: 16.971
  2 in total

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