Literature DB >> 9278236

The nuclear membrane integrity assay.

M P Czubryt1, B Ramjiawan, G N Pierce.   

Abstract

A novel technique is described for the evaluation of membrane integrity in isolated nuclei. Membrane integrity is assessed by measuring nuclear fragility in response to high salt conditions. Salt-induced disruption of the nuclear membrane is followed by spectrophotometric monitoring of released nucleotides. The assay is based on determining the amount of salt necessary to induce release of 50% of the total pool of releasable nucleotides. This allows semiquantitative comparison of relative nuclear membrane strength or integrity of different samples in response to high salt conditions. In this manner, the effects of altered nuclear membrane composition or metabolism on membrane integrity may be monitored.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9278236

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  15 in total

1.  Cholesterol modulates activity of calcium-dependent ATPase of the sarcoplasmic reticulum.

Authors:  T D Madden; D Chapman; P J Quinn
Journal:  Nature       Date:  1979-06-07       Impact factor: 49.962

2.  Improved microfluorometric DNA determination in biological material using 33258 Hoechst.

Authors:  C F Cesarone; C Bolognesi; L Santi
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

3.  Quantitation of protein.

Authors:  C M Stoscheck
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

4.  Protein determination in membrane and lipoprotein samples: manual and automated procedures.

Authors:  M A Markwell; S M Haas; N E Tolbert; L L Bieber
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

5.  Adaptive changes in subcellular calcium transport during catecholamine-induced cardiomyopathy.

Authors:  V Panagia; G N Pierce; K S Dhalla; P K Ganguly; R E Beamish; N S Dhalla
Journal:  J Mol Cell Cardiol       Date:  1985-04       Impact factor: 5.000

6.  Nuclear cholesterol content and nucleoside triphosphatase activity are altered in the JCR:LA-cp corpulent rat.

Authors:  M P Czubryt; J C Russell; J Sarantopoulos; G N Pierce
Journal:  J Cell Biochem       Date:  1996-12-01       Impact factor: 4.429

7.  Alterations in Ca2+ binding by and composition of the cardiac sarcolemmal membrane in chronic diabetes.

Authors:  G N Pierce; M J Kutryk; N S Dhalla
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Sarcolemmal Na+-K+-ATPase activity in diabetic rat heart.

Authors:  G N Pierce; N S Dhalla
Journal:  Am J Physiol       Date:  1983-09

9.  Identification and purification of a calcium-binding protein in hepatic nuclear membranes.

Authors:  J S Gilchrist; G N Pierce
Journal:  J Biol Chem       Date:  1993-02-25       Impact factor: 5.157

Review 10.  Organization and function of sarcolemmal phospholipids in control and ischemic/reperfused cardiomyocytes.

Authors:  J A Post; A J Verkleij; G A Langer
Journal:  J Mol Cell Cardiol       Date:  1995-02       Impact factor: 5.000
View more
  3 in total

1.  Age- and sex-related differences in nuclear lipid content and nucleoside triphosphatase activity in the JCR:LA-cp corpulent rat.

Authors:  M P Czubryt; J C Russell; J Sarantopoulos; J S Gilchrist; G N Pierce
Journal:  Mol Cell Biochem       Date:  1997-11       Impact factor: 3.396

2.  Increased expression of peripheral benzodiazepine receptor (PBR) in dimethylbenz[a]anthracene-induced mammary tumors in rats.

Authors:  Sutapa Mukhopadhyay; Shyamali Mukherjee; Salil K Das
Journal:  Glycoconj J       Date:  2006-05       Impact factor: 2.916

3.  The mechanistic role of alpha-synuclein in the nucleus: impaired nuclear function caused by familial Parkinson's disease SNCA mutations.

Authors:  Vivian Chen; Malik Moncalvo; Dominic Tringali; Lidia Tagliafierro; Ahila Shriskanda; Ekaterina Ilich; Wendy Dong; Boris Kantor; Ornit Chiba-Falek
Journal:  Hum Mol Genet       Date:  2020-11-04       Impact factor: 6.150
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.