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Literature DB >> 9276434

A new experimental in vitro culture medium for cultivation of Leishmania species.

M E Limoncu¹, I C Balcioğlu, K Yereli, Y Ozbel, A Ozbilgin.

Abstract

A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 10(7)/ml at the end of the 8th day in our new medium, though in NNN medium the number of organisms reached only 5 x 10(6)/ml. After 10 subsequent passages, the culture medium prepared was evaluated as being quite inexpensive, simple, and successful compared with other commercially available liquid culture media.

Entities: Disease

Mesh：

Substances：
Culture Media

Year: 1997 PMID： 9276434 PMCID： PMC229986 DOI： 10.1128/jcm.35.9.2430-2431.1997

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

5 in total

1. Leishmania donovani: use of two different, commercially available, chemically defined media for the continuous in vitro cultivation of promastigotes.

Authors: C McCarthy-Burke; P A Bates; D M Dwyer
Journal: Exp Parasitol Date: 1991-10 Impact factor: 2.011

2. A new liquid medium without blood and serum for culture of hemoflagellates.

Authors: M Sadigursky; C I Brodskyn
Journal: Am J Trop Med Hyg Date: 1986-09 Impact factor: 2.345

3. A simple monophasic medium for axenic culture of hemoflagellates.

Authors: R L Berens; R Brun; S M Krassner
Journal: J Parasitol Date: 1976-06 Impact factor: 1.276

4. Axenic cultivation of amastigotes of Leishmania donovani and Leishmania major and their infectivity.

Authors: N T al-Bashir; M B Rassam; B M al-Rawi
Journal: Ann Trop Med Parasitol Date: 1992-10

5. Carbon dioxide abolishes the reverse Pasteur effect in Leishmania major promastigotes.

Authors: T N Darling; D G Davis; R E London; J J Blum
Journal: Mol Biochem Parasitol Date: 1989-03-01 Impact factor: 1.759

5 in total

6 in total

1. Antibodies against Leishmania cross-react with Crithidia luciliae: indirect immunofluorescence and Dot-ELISA study in dogs.

Authors: Franjo Martinkovic; Albert Marinculic
Journal: Parasitol Res Date: 2005-12-13 Impact factor: 2.289

2. An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human Erythrocytes.

Authors: Pallavi Singh; Anasuya C Pal; Choukri Ben Mamoun
Journal: Pathogens Date: 2022-05-20

A new experimental in vitro culture medium for cultivation of Leishmania species.

1. Leishmania donovani: use of two different, commercially available, chemically defined media for the continuous in vitro cultivation of promastigotes.

2. A new liquid medium without blood and serum for culture of hemoflagellates.

3. A simple monophasic medium for axenic culture of hemoflagellates.

4. Axenic cultivation of amastigotes of Leishmania donovani and Leishmania major and their infectivity.

5. Carbon dioxide abolishes the reverse Pasteur effect in Leishmania major promastigotes.

1. Antibodies against Leishmania cross-react with Crithidia luciliae: indirect immunofluorescence and Dot-ELISA study in dogs.

2. An Alternative Culture Medium for Continuous In Vitro Propagation of the Human Pathogen Babesia duncani in Human Erythrocytes.

Review 3. Recombinant protein expression in Leishmania tarentolae.

4. Low-cost liquid medium for in vitro cultivation of Leishmania parasites in low-income countries.

Review 5. Cultivation of clinically significant hemoflagellates.

6. A simple monophasic LGPY medium for routine maintenance of Leishmania donovani promastigotes.