Characterization of functional interaction of carboxylic acid group of agonists and arginine of the seventh transmembrane domains of four prostaglandin E receptor subtypes.

Prostaglandin (PG) E2 binds to four PGE receptor subtypes, EP1, EP2, EP3 and EP4, and induces a variety of functions through the interaction of carboxylic acid of PGE2 and Arg residue in the seventh transmembrane domain of the receptor. To assess the role of the interaction of the carboxylic acid group of agonists and the Arg residue, which can form both ionic bonding and hydrogen bonding as a hydrogen donor, we examined the agonist activities of three types of agonist, PGE2 with a negatively charged carboxylic acid, PGE2 methylester, which is a hydrogen acceptor, and 1-OH PGE2, which can accept as well as donate hydrogen but prefers to donate hydrogen rather than accept it, for four PGE receptor subtypes. Although PGE2 methylester had slightly lower agonist activities than PGE2 for EP1 and EP4 receptors, PGE2 and its methylester showed the same agonist activities for EP2 and EP3 receptors, indicating that PGE2 methylester is a potent agonist for all of the four subtypes. In contrast, 1-OH PGE2 was a very weak agonist for all receptors. These findings demonstrate that the hydrogen bonding interaction of agonists and the Arg residue is generally sufficient for the functional activation of all of the PGE receptor subtypes.