| Literature DB >> 927050 |
T N Seyfried, E J Weber, R K Yu.
Abstract
Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.Entities:
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Year: 1977 PMID： 927050 DOI： 10.1007/bf02533321
Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880