UNLABELLED: Anti-endomysium antibody (AEA) was evaluated in 136 subjects by indirect immunofluorescence using both cryosections of monkey oesophagus (MO) and the human umbilical cord vein (HUCV) as substrate. This human tissue gave results comparable to those of MO. In particular, the HUCV sections showed positive results in all 22 newly diagnosed cases of coeliac disease. Compared to the MO sections, the sensitivity, specificity and positive predictive values of HUCV tissue was 100%. CONCLUSION: HUCV tissue can replace MO for AEA detection and can make the screening for coeliac disease easier both in at-risk and in normal populations, with a remarkable saving, both in terms of money and of monkeys.
UNLABELLED: Anti-endomysium antibody (AEA) was evaluated in 136 subjects by indirect immunofluorescence using both cryosections of monkey oesophagus (MO) and the human umbilical cord vein (HUCV) as substrate. This human tissue gave results comparable to those of MO. In particular, the HUCV sections showed positive results in all 22 newly diagnosed cases of coeliac disease. Compared to the MO sections, the sensitivity, specificity and positive predictive values of HUCV tissue was 100%. CONCLUSION: HUCV tissue can replace MO for AEA detection and can make the screening for coeliac disease easier both in at-risk and in normal populations, with a remarkable saving, both in terms of money and of monkeys.
Authors: L Nisticò; C Fagnani; I Coto; S Percopo; R Cotichini; M G Limongelli; F Paparo; S D'Alfonso; M Giordano; C Sferlazzas; G Magazzù; P Momigliano-Richiardi; L Greco; M A Stazi Journal: Gut Date: 2005-12-14 Impact factor: 23.059
Authors: I Berti; C Trevisiol; A Tommasini; A Città; E Neri; O Geatti; A Giammarini; A Ventura; T Not Journal: Dig Dis Sci Date: 2000-02 Impact factor: 3.199
Authors: V Baldas; A Tommasini; C Trevisiol; I Berti; A Fasano; D Sblattero; A Bradbury; R Marzari; G Barillari; A Ventura; T Not Journal: Gut Date: 2000-11 Impact factor: 23.059
Authors: F Ferrara; S Quaglia; I Caputo; C Esposito; M Lepretti; S Pastore; R Giorgi; S Martelossi; G Dal Molin; N Di Toro; A Ventura; T Not Journal: Clin Exp Immunol Date: 2009-11-12 Impact factor: 4.330