C Kee1, K Seo. 1. Department of Ophthalmology, Samsung Medical Center, College of Medicine, Sungkyunkwan University, Seoul, Korea.
Abstract
PURPOSE: The authors investigated whether regulation of the trabecular extracellular matrix turnover rate and remodeling plays an important role in decreasing outflow resistance by determining the effect of intracamerally given interleukin-1alpha, a known stimulator of the expression of trabecular matrix metalloproteinases, on outflow facility of albino rat eyes. MATERIALS AND METHODS: Forty normal albino rats (Sprague-Dawley), weighing 250 to 300 g, were studied. Rats were anesthetized by intraperitoneal pentobarbital sodium (30 mg/kg) injection. The rats were separated into four groups and given 5, 10, 25, or 50 units of interleukin-1alpha injected intracamerally in one eye. Bovine serum albumin in phosphate-buffered saline, which was used to dissolve the interleukin-1alpha, was injected in the fellow eye as a control. Outflow facility was measured by two-level constant pressure perfusion 1, 3, and 7 days after injection. RESULTS: The eyes treated with 50 units of interleukin-1alpha showed a statistically significant increase of outflow facility by 37% compared with the contralateral control eyes 3 days after injection, but returned to normal levels in 7 days. The eyes treated with 5, 10, 25, or 50 units of interleukin-1alpha showed outflow facility increases of 12%, 20%, 39%, and 37%, respectively, 3 days after injection. CONCLUSION: Interleukin-1alpha increased the outflow facility, supporting the hypothesis that regulation of trabecular meshwork extracellular matrix plays a role in trabecular outflow resistance.
PURPOSE: The authors investigated whether regulation of the trabecular extracellular matrix turnover rate and remodeling plays an important role in decreasing outflow resistance by determining the effect of intracamerally given interleukin-1alpha, a known stimulator of the expression of trabecular matrix metalloproteinases, on outflow facility of albino rat eyes. MATERIALS AND METHODS: Forty normal albino rats (Sprague-Dawley), weighing 250 to 300 g, were studied. Rats were anesthetized by intraperitoneal pentobarbital sodium (30 mg/kg) injection. The rats were separated into four groups and given 5, 10, 25, or 50 units of interleukin-1alpha injected intracamerally in one eye. Bovineserum albumin in phosphate-buffered saline, which was used to dissolve the interleukin-1alpha, was injected in the fellow eye as a control. Outflow facility was measured by two-level constant pressure perfusion 1, 3, and 7 days after injection. RESULTS: The eyes treated with 50 units of interleukin-1alpha showed a statistically significant increase of outflow facility by 37% compared with the contralateral control eyes 3 days after injection, but returned to normal levels in 7 days. The eyes treated with 5, 10, 25, or 50 units of interleukin-1alpha showed outflow facility increases of 12%, 20%, 39%, and 37%, respectively, 3 days after injection. CONCLUSION:Interleukin-1alpha increased the outflow facility, supporting the hypothesis that regulation of trabecular meshwork extracellular matrix plays a role in trabecular outflow resistance.
Authors: Mariana B Oliveira; José Paulo C de Vasconcellos; Galina Ananina; Vital P Costa; Mônica B de Melo Journal: Exp Biol Med (Maywood) Date: 2018-09
Authors: M Elizabeth Fini; Stephen G Schwartz; Xiaoyi Gao; Shinwu Jeong; Nitin Patel; Tatsuo Itakura; Marianne O Price; Francis W Price; Rohit Varma; W Daniel Stamer Journal: Prog Retin Eye Res Date: 2016-09-22 Impact factor: 21.198