Literature DB >> 9259473

Destruction of neurokinin-1 receptor expressing cells in vitro and in vivo using substance P-saporin in rats.

R G Wiley1, D A Lappi.   

Abstract

Substance P (SP) acts on neurons through the neurokinin-1 (NK-1) receptor. Conjugation of SP to the ribosome inactivating protein, saporin (SAP), produces a cytotoxin selective for cells that express the NK-1 receptor. SP-SAP cytotoxicity was inhibited by pre-treating the toxin to reduce the disulfide bond connecting SP to SAP or by pre-incubation with anti-SP antiserum or by SP analog showing that SP-SAP acts through binding of the SP moiety to NK-1 receptors. Injection of SP-SAP into the striatum selectively destroyed NK-1 receptor expressing interneurons. These results show that SP-SAP will be useful for studying the function of NK-1 receptor expressing neurons.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9259473     DOI： 10.1016/s0304-3940(97)00490-4

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  19 in total

1.  Depletion of cholinergic amacrine cells by a novel immunotoxin does not perturb the formation of segregated on and off cone bipolar cell projections.

Authors:  Emine Gunhan; Prabhakara V Choudary; Thomas E Landerholm; Leo M Chalupa
Journal:  J Neurosci       Date:  2002-03-15       Impact factor: 6.167

2.  Impairment of skilled forelimb use after ablation of striatal interneurons expressing substance P receptors in rats: an analysis using a pasta matrix reaching task.

Authors:  Satomi Chiken; Hironobu Tokuno
Journal:  Exp Brain Res       Date:  2005-03-08       Impact factor: 1.972

3.  Characteristics of substance P transport across the blood-brain barrier.

Authors:  Arvind K Chappa; Kenneth L Audus; Susan M Lunte
Journal:  Pharm Res       Date:  2006-06-01       Impact factor: 4.200

4.  Substance P-saporin lesion of neurons with NK1 receptors in one chemoreceptor site in rats decreases ventilation and chemosensitivity.

Authors:  Eugene E Nattie; Aihua Li
Journal:  J Physiol       Date:  2002-10-15       Impact factor: 5.182

5.  Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control.

Authors:  Li-Hsien Lin; Steven A Moore; Susan Y Jones; Jacob McGlashon; William T Talman
Journal:  J Neurosci       Date:  2013-11-20       Impact factor: 6.167

6.  Hypocretin-2-saporin lesions of the lateral hypothalamus produce narcoleptic-like sleep behavior in the rat.

Authors:  D Gerashchenko; M D Kohls; M Greco; N S Waleh; R Salin-Pascual; T S Kilduff; D A Lappi; P J Shiromani
Journal:  J Neurosci       Date:  2001-09-15       Impact factor: 6.167

7.  Collateral damage and compensatory changes after injection of a toxin targeting neurons with the neurokinin-1 receptor in the nucleus tractus solitarii of rat.

Authors:  Li-Hsien Lin; Deidre Nitschke Dragon; William T Talman
Journal:  J Chem Neuroanat       Date:  2012-03-06       Impact factor: 3.052

8.  Ventilatory effects of substance P-saporin lesions in the nucleus tractus solitarii of chronically hypoxic rats.

Authors:  Katherine A Wilkinson; Zhenxing Fu; Frank L Powell
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2011-05-18       Impact factor: 3.619

9.  Medullary serotonergic neurones and adjacent neurones that express neurokinin-1 receptors are both involved in chemoreception in vivo.

Authors:  Eugene E Nattie; Aihua Li; George B Richerson; George Richerson; Douglas A Lappi
Journal:  J Physiol       Date:  2004-01-14       Impact factor: 5.182

10.  Neurokinin-1 receptor-expressing neurons in the amygdala modulate morphine reward and anxiety behaviors in the mouse.

Authors:  Christopher A Gadd; Patricia Murtra; Carmen De Felipe; Stephen P Hunt
Journal:  J Neurosci       Date:  2003-09-10       Impact factor: 6.167
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.