OBJECTIVE: To establish a novel 3-dimensional (3-D) in vitro model for the investigation of destructive processes in rheumatoid arthritis (RA). METHODS: Two distinct culture systems were developed, consisting of RA synovial membrane and articular cartilage explants or interactive RA synovial cell/chondrocyte cultures embedded in 3-D fibrin matrices. The expression of proteolytic enzymes, chondrocyte matrix architecture, and matrix degradation parameters was analyzed by immunohistochemistry. RESULTS: Of 28 RA explant cultures, 16 displayed an invasion of synovial tissue into the cartilage explants, compared with 1 of 8 osteoarthritis explants. The expression of collagenase and vascular cell adhesion molecule 1 could be demonstrated at the cartilage-pannus junction. Of 20 interactive cell cultures, 18 revealed invasive behavior and remained vital for extended periods of time. CONCLUSION: The models presented allow us to study distinct aspects of destructive joint diseases under in vitro conditions that resemble human pathology. Moreover, our model is able to supplement animal experiments in basic research and drug testing.
OBJECTIVE: To establish a novel 3-dimensional (3-D) in vitro model for the investigation of destructive processes in rheumatoid arthritis (RA). METHODS: Two distinct culture systems were developed, consisting of RA synovial membrane and articular cartilage explants or interactive RA synovial cell/chondrocyte cultures embedded in 3-D fibrin matrices. The expression of proteolytic enzymes, chondrocyte matrix architecture, and matrix degradation parameters was analyzed by immunohistochemistry. RESULTS: Of 28 RA explant cultures, 16 displayed an invasion of synovial tissue into the cartilage explants, compared with 1 of 8 osteoarthritis explants. The expression of collagenase and vascular cell adhesion molecule 1 could be demonstrated at the cartilage-pannus junction. Of 20 interactive cell cultures, 18 revealed invasive behavior and remained vital for extended periods of time. CONCLUSION: The models presented allow us to study distinct aspects of destructive joint diseases under in vitro conditions that resemble human pathology. Moreover, our model is able to supplement animal experiments in basic research and drug testing.
Authors: T Zimmermann; E Kunisch; R Pfeiffer; A Hirth; H D Stahl; U Sack; A Laube; E Liesaus; A Roth; E Palombo-Kinne; F Emmrich; R W Kinne Journal: Arthritis Res Date: 2000-11-21
Authors: Stine Mandrup Andreassen; Anne Mette Lindberg Vinther; Søren Saxmose Nielsen; Pia Haubro Andersen; Aziz Tnibar; Annemarie T Kristensen; Stine Jacobsen Journal: BMC Vet Res Date: 2017-06-19 Impact factor: 2.741
Authors: Martin Andersen; Mikael Boesen; Karen Ellegaard; Kalle Söderström; Niels H Søe; Pieter Spee; Ulrik G W Mørch; Søren Torp-Pedersen; Else M Bartels; Bente Danneskiold-Samsøe; Lars Karlsson; Henning Bliddal Journal: PLoS One Date: 2018-05-22 Impact factor: 3.240