Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein.

Literature DB >> 9258446

Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein.

Abstract

An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. 6-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.

Entities: Chemical

Mesh：

Substances：

Year: 1997 PMID： 9258446 DOI： 10.1021/bc970078d

Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774

Keyword Cloud
Cited

6 in total

Review 1. Green and Red Fluorescent Dyes for Translational Applications in Imaging and Sensing Analytes: A Dual-Color Flag.

Authors: Elisabete Oliveira; Emilia Bértolo; Cristina Núñez; Viviane Pilla; Hugo M Santos; Javier Fernández-Lodeiro; Adrian Fernández-Lodeiro; Jamila Djafari; José Luis Capelo; Carlos Lodeiro
Journal: ChemistryOpen Date: 2017-11-07 Impact factor: 2.911

2. Synthesis, spectroscopic properties, and biological applications of eight novel chlorinated fluorescent proteins-labeling probes.

Authors: Xianglong Wu; Min Tian; Wutu Fan; Yalei Pan; Yuankun Zhai; Yinbo Niu; Chenrui Li; Tingli Lu; Qibing Mei
Journal: J Fluoresc Date: 2014-02-04 Impact factor: 2.217

6. Separating the isomers--efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B.

Authors: Aurélie Brunet; Tashfeen Aslam; Mark Bradley
Journal: Bioorg Med Chem Lett Date: 2014-05-04 Impact factor: 2.823

6 in total

Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein.

Review 1. Green and Red Fluorescent Dyes for Translational Applications in Imaging and Sensing Analytes: A Dual-Color Flag.

2. Synthesis, spectroscopic properties, and biological applications of eight novel chlorinated fluorescent proteins-labeling probes.

3. New fluorescent labels: 4- and 7-chlorofluorescein.

4. Peptide Targeting of Fluorescein-Based Sensors to Discrete Intracellular Locales.

5. Synthesis of rhodamines from fluoresceins using Pd-catalyzed C-N cross-coupling.

6. Separating the isomers--efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B.