C Y Wang1, Y W Chang. 1. Department of Urology, State University of New York Health Science Center, Syracuse 13210, USA.
Abstract
BACKGROUND: A nude mouse model was developed and studied for temporal pathogenesis of bony metastasis of human breast cancer. MATERIALS AND METHODS: Human breast cancer cells, MDA-MB-435, were injected into the medulla of left femurs of 10 Beige nude mice, whereas their right femurs were similarly injected with phosphate buffered saline as a control. The mice were killed at 1 week-intervals for up to 5 weeks. RESULTS: Tumors appeared in all the animals 3 weeks after the injection: they began to appear in the medulla at 2 weeks, and invasion of the tumor into the cortex and surrounding tissues of the bone appeared at 4 weeks. Both osteolytic and osteoblastic activation frequently observed in the affected bone of breast cancer patients were present. CONCLUSION: This model produces a high incidence of bony metastasis and may be appropriate for the study of osseous metastasis of this human disease.
BACKGROUND: A nude mouse model was developed and studied for temporal pathogenesis of bony metastasis of humanbreast cancer. MATERIALS AND METHODS:Humanbreast cancer cells, MDA-MB-435, were injected into the medulla of left femurs of 10 Beige nude mice, whereas their right femurs were similarly injected with phosphate buffered saline as a control. The mice were killed at 1 week-intervals for up to 5 weeks. RESULTS:Tumors appeared in all the animals 3 weeks after the injection: they began to appear in the medulla at 2 weeks, and invasion of the tumor into the cortex and surrounding tissues of the bone appeared at 4 weeks. Both osteolytic and osteoblastic activation frequently observed in the affected bone of breast cancerpatients were present. CONCLUSION: This model produces a high incidence of bony metastasis and may be appropriate for the study of osseous metastasis of this human disease.
Authors: Antoinette Wetterwald; Gabri van der Pluijm; Ivo Que; Bianca Sijmons; Jeroen Buijs; Marcel Karperien; Clemens W G M Löwik; Elsbeth Gautschi; George N Thalmann; Marco G Cecchini Journal: Am J Pathol Date: 2002-03 Impact factor: 4.307
Authors: S Cowey; A A Szafran; J Kappes; K R Zinn; G P Siegal; R A Desmond; H Kim; L Evans; R W Hardy Journal: Clin Exp Metastasis Date: 2007-05-31 Impact factor: 5.150