Literature DB >> 9245611

In vitro assays for activities of retroviral integrase.

S A Chow1.   

Abstract

Integration of retroviral DNA, an essential step during the retroviral life cycle, is mediated by the viral protein integrase. Simple in vitro assays for measuring integrase activities are described, including catalysis (3'-end processing, 3'-end joining, disintegration), juxtaposition of viral DNA ends, DNA binding, and target site selection. The described assays will be useful in elucidating the molecular mechanism of retroviral integration and screening for integrase inhibitors as potential anti-retroviral drugs.

Mesh：

Substances：

Year:  1997        PMID： 9245611     DOI： 10.1006/meth.1997.0484

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  20 in total

1.  Inhibition of the integrases of human immunodeficiency viruses type 1 and type 2 by reverse transcriptases.

Authors:  Iris Oz; Orna Avidan; Amnon Hizi
Journal:  Biochem J       Date:  2002-02-01       Impact factor: 3.857

2.  Inhibition of the activity of recombinant HIV-1 integrase by derivatives of higher terpenoids.

Authors:  E A Semenova; O A Plyasunova; N I Petrenko; N V Uzenkova; E E Shul'ts; G A Tolstikov; A G Pokrovskii
Journal:  Dokl Biochem Biophys       Date:  2003 Jul-Aug       Impact factor: 0.788

3.  Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication.

Authors:  Shewit S Tekeste; Thomas A Wilkinson; Ethan M Weiner; Xiaowen Xu; Jennifer T Miller; Stuart F J Le Grice; Robert T Clubb; Samson A Chow
Journal:  J Virol       Date:  2015-09-23       Impact factor: 5.103

4.  Preliminary mapping of a putative inhibitor-binding pocket for human immunodeficiency virus type 1 integrase inhibitors.

Authors:  Deborah J Lee; W Edward Robinson
Journal:  Antimicrob Agents Chemother       Date:  2006-01       Impact factor: 5.191

5.  Identification of a nucleotide binding site in HIV-1 integrase.

Authors:  R R Drake; N Neamati; H Hong; A A Pilon; P Sunthankar; S D Hume; G W Milne; Y Pommier
Journal:  Proc Natl Acad Sci U S A       Date:  1998-04-14       Impact factor: 11.205

6.  A three-dimensional model of the human immunodeficiency virus type 1 integration complex.

Authors:  Jerome Wielens; Ian T Crosby; David K Chalmers
Journal:  J Comput Aided Mol Des       Date:  2005-05       Impact factor: 3.686

7.  Requirement for integrase during reverse transcription of human immunodeficiency virus type 1 and the effect of cysteine mutations of integrase on its interactions with reverse transcriptase.

Authors:  Kai Zhu; Charles Dobard; Samson A Chow
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

8.  Integrase-specific enhancement and suppression of retroviral DNA integration by compacted chromatin structure in vitro.

Authors:  Konstantin D Taganov; Isabel Cuesta; René Daniel; Lisa Ann Cirillo; Richard A Katz; Kenneth S Zaret; Anna Marie Skalka
Journal:  J Virol       Date:  2004-06       Impact factor: 5.103

9.  Human immunodeficiency virus type 1 (HIV-1) integrase: resistance to diketo acid integrase inhibitors impairs HIV-1 replication and integration and confers cross-resistance to L-chicoric acid.

Authors:  Deborah J Lee; W E Robinson
Journal:  J Virol       Date:  2004-06       Impact factor: 5.103

10.  Fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc finger protein E2C direct integration of viral DNA into specific sites.

Authors:  Wenjie Tan; Kai Zhu; David J Segal; Carlos F Barbas; Samson A Chow
Journal:  J Virol       Date:  2004-02       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.