OBJECTIVE: The aim of the present study was to investigate the presence of interleukin-8 (IL-8) in the human cervix and whether the levels of interleukin-8 could be related to the ripening process during pregnancy. STUDY DESIGN: Cervical biopsies were obtained in twelve term pregnant and in eight vaginally delivered women. Seven non-pregnant fertile women served as controls. After homogenisation and centrifugation, IL-8 levels were determined in the supernatant by an enzyme-immunoassay (EIA). RESULTS: In women at term, the concentration of IL-8 increased six-fold from median 330 pg/ml to median 2190 pg/ml (P < 0.001). After the final cervical ripening it increased in additional 11-fold to median 26,100 pg/ml (P < 0.001). These changes are highly significant. CONCLUSION: To our knowledge, this is the first time IL-8 has been identified in human cervix. Our results support the involvement of IL-8 in the connective tissue remodelling during the final cervical ripening just before onset of labour.
OBJECTIVE: The aim of the present study was to investigate the presence of interleukin-8 (IL-8) in the human cervix and whether the levels of interleukin-8 could be related to the ripening process during pregnancy. STUDY DESIGN: Cervical biopsies were obtained in twelve term pregnant and in eight vaginally delivered women. Seven non-pregnant fertile women served as controls. After homogenisation and centrifugation, IL-8 levels were determined in the supernatant by an enzyme-immunoassay (EIA). RESULTS: In women at term, the concentration of IL-8 increased six-fold from median 330 pg/ml to median 2190 pg/ml (P < 0.001). After the final cervical ripening it increased in additional 11-fold to median 26,100 pg/ml (P < 0.001). These changes are highly significant. CONCLUSION: To our knowledge, this is the first time IL-8 has been identified in human cervix. Our results support the involvement of IL-8 in the connective tissue remodelling during the final cervical ripening just before onset of labour.
Authors: Pooja Mittal; Roberto Romero; Adi L Tarca; Sorin Draghici; Chia-Ling Nhan-Chang; Tinnakorn Chaiworapongsa; John Hotra; Ricardo Gomez; Juan Pedro Kusanovic; Deug-Chan Lee; Chong Jai Kim; Sonia S Hassan Journal: Am J Obstet Gynecol Date: 2011-02 Impact factor: 8.661
Authors: Sonia S Hassan; Roberto Romero; Adi L Tarca; Sorin Draghici; Beth Pineles; Andrej Bugrim; Nahla Khalek; Natalia Camacho; Pooja Mittal; Bo Hyun Yoon; Jimmy Espinoza; Chong Jai Kim; Yoram Sorokin; John Malone Journal: Am J Obstet Gynecol Date: 2007-09 Impact factor: 8.661
Authors: Pooja Mittal; Roberto Romero; Adi L Tarca; Juan Gonzalez; Sorin Draghici; Yi Xu; Zhong Dong; Chia-Ling Nhan-Chang; Tinnakorn Chaiworapongsa; Stephen Lye; Juan Pedro Kusanovic; Leonard Lipovich; Shali Mazaki-Tovi; Sonia S Hassan; Sam Mesiano; Chong Jai Kim Journal: J Perinat Med Date: 2010-07-14 Impact factor: 1.901
Authors: Sonia S Hassan; Roberto Romero; Adi L Tarca; Chia-Ling Nhan-Chang; Edi Vaisbuch; Offer Erez; Pooja Mittal; Juan Pedro Kusanovic; Shali Mazaki-Tovi; Lami Yeo; Sorin Draghici; Jung-Sun Kim; Niels Uldbjerg; Chong Jai Kim Journal: J Matern Fetal Neonatal Med Date: 2009-12
Authors: Pooja Mittal; Roberto Romero; Juan Pedro Kusanovic; Samuel S Edwin; Francesca Gotsch; Shali Mazaki-Tovi; Jimmy Espinoza; Offer Erez; Chia-Ling Nhan-Chang; Nandor G Than; Edi Vaisbuch; Sonia S Hassan Journal: Am J Reprod Immunol Date: 2008-09 Impact factor: 3.886
Authors: A Dubicke; A Akerud; M Sennstrom; R Rafik Hamad; B Bystrom; A Malmstrom; G Ekman-Ordeberg Journal: Mol Hum Reprod Date: 2008-10-15 Impact factor: 4.025