Literature DB >> 9241256

Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions.

D M Kube1, A Srivastava.   

Abstract

Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg2+ions also interfered with the adsorption of plasmid DNA to nylon and nitrocellulose membranes. These observations yield practical insights into the poorly understood mechanisms by which DNA molecules are retained on solid supports.

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Year:  1997        PMID： 9241256      PMCID： PMC146885          DOI： 10.1093/nar/25.16.3375

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  A method for the detection of RNA-DNA complexes.

Authors:  A P NYGAARD; B D HALL
Journal:  Biochem Biophys Res Commun       Date:  1963-07-18       Impact factor: 3.575

Review 2.  RNA fractionation on modified celluloses. I. Ecteola-, ectham-, amino-ethyl-, nucleic acid-, and nitro-cellulose.

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Authors:  A P Phillips
Journal:  Biochim Biophys Acta       Date:  1969-11-19

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Authors:  A Douvas; P A Price
Journal:  Biochim Biophys Acta       Date:  1975-06-16

Review 5.  Hybridization of nucleic acids immobilized on solid supports.

Authors:  J Meinkoth; G Wahl
Journal:  Anal Biochem       Date:  1984-05-01       Impact factor: 3.365

6.  Rapid transfer of DNA from agarose gels to nylon membranes.

Authors:  K C Reed; D A Mann
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

7.  The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.

Authors:  I Gillam; S Millward; D Blew; M von Tigerstrom; E Wimmer; G M Tener
Journal:  Biochemistry       Date:  1967-10       Impact factor: 3.162

8.  Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression.

Authors:  R J Samulski; L S Chang; T Shenk
Journal:  J Virol       Date:  1989-09       Impact factor: 5.103

9.  Cloning of adeno-associated virus into pBR322: rescue of intact virus from the recombinant plasmid in human cells.

Authors:  R J Samulski; K I Berns; M Tan; N Muzyczka
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205
  9 in total
  22 in total

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Authors:  J Hansen; K Qing; A Srivastava
Journal:  J Virol       Date:  2001-05       Impact factor: 5.103

2.  High-efficiency transduction of fibroblasts and mesenchymal stem cells by tyrosine-mutant AAV2 vectors for their potential use in cellular therapy.

Authors:  Mengxin Li; Giridhara R Jayandharan; Baozheng Li; Chen Ling; Wenqin Ma; Arun Srivastava; Li Zhong
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3.  Characterization of wild-type adeno-associated virus type 2-like particles generated during recombinant viral vector production and strategies for their elimination.

Authors:  X S Wang; B Khuntirat; K Qing; S Ponnazhagan; D M Kube; S Zhou; V J Dwarki; A Srivastava
Journal:  J Virol       Date:  1998-07       Impact factor: 5.103

4.  Rescue and autonomous replication of adeno-associated virus type 2 genomes containing Rep-binding site mutations in the viral p5 promoter.

Authors:  X S Wang; A Srivastava
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

5.  Limitations of encapsidation of recombinant self-complementary adeno-associated viral genomes in different serotype capsids and their quantitation.

Authors:  Yuan Wang; Chen Ling; Liujiang Song; Lina Wang; George V Aslanidi; Mengqun Tan; Changquan Ling; Arun Srivastava
Journal:  Hum Gene Ther Methods       Date:  2012-08       Impact factor: 2.396

6.  Bioengineering of AAV2 capsid at specific serine, threonine, or lysine residues improves its transduction efficiency in vitro and in vivo.

Authors:  Nishanth Gabriel; Sangeetha Hareendran; Dwaipayan Sen; Rupali A Gadkari; Govindarajan Sudha; Ruchita Selot; Mansoor Hussain; Ramya Dhaksnamoorthy; Rekha Samuel; Narayanaswamy Srinivasan; Alok Srivastava; Giridhara R Jayandharan
Journal:  Hum Gene Ther Methods       Date:  2013-03-15       Impact factor: 2.396

7.  Recombinant human parvovirus B19 vectors: erythroid cell-specific delivery and expression of transduced genes.

Authors:  S Ponnazhagan; K A Weigel; S P Raikwar; P Mukherjee; M C Yoder; A Srivastava
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

8.  Targeted modifications in adeno-associated virus serotype 8 capsid improves its hepatic gene transfer efficiency in vivo.

Authors:  Dwaipayan Sen; Rupali A Gadkari; Govindarajan Sudha; Nishanth Gabriel; Yesupatham Sathish Kumar; Ruchita Selot; Rekha Samuel; Sumathi Rajalingam; V Ramya; Sukesh C Nair; Narayanaswamy Srinivasan; Alok Srivastava; Giridhara R Jayandharan
Journal:  Hum Gene Ther Methods       Date:  2013-04       Impact factor: 2.396

9.  Optimized adeno-associated virus (AAV)-protein phosphatase-5 helper viruses for efficient liver transduction by single-stranded AAV vectors: therapeutic expression of factor IX at reduced vector doses.

Authors:  Giridhara R Jayandharan; Li Zhong; Brandon K Sack; Angela E Rivers; Mengxin Li; Baozheng Li; Roland W Herzog; Arun Srivastava
Journal:  Hum Gene Ther       Date:  2010-03       Impact factor: 5.695

10.  Gene transfer in the liver using recombinant adeno-associated virus.

Authors:  Seemin Seher Ahmed; Jia Li; Jonathan Godwin; Guangping Gao; Li Zhong
Journal:  Curr Protoc Microbiol       Date:  2013
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