A neuroanatomical method to assess the integrity of fibers of passage following ibotenate-induced damage to the central nervous system.

In some behavioral-lesion experiments involving animals, ibotenic acid (IBO) is used as a means of damaging brain structures. Occasionally one needs to assess the status of fibers coursing through the damaged area to determine whether the deficits observed are the result of destruction of neurons rather than fibers. In such cases, IBO, is considered to be the method of choice since it destroys cell bodies but leaves fibers of passage intact. However, if the IBO dose injected in a given area is too high, both cell bodies and fibers of passage could be damaged. The anterograde and retrograde tracer wheat germ agglutinin-horseradish peroxidase (WGA-HRP) is a useful technique to verify that fibers are intact, and is a more powerful tool in comparison with a tracer, such as HRP, which has been used in previous studies.