Literature DB >> 9234816

Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model.

J A Bénitez1, R G Spelbrink, A Silva, T E Phillips, C M Stanley, M Boesman-Finkelstein, R A Finkelstein.   

Abstract

Choleragenic vibrios adhered to and multiplied on monolayers of the highly differentiated mucin-secreting cell line HT29-18N2. Their adherence followed first-order kinetics, was dependent on the concentration of vibrios, and was partially inhibited by lipopolysaccharide. Comparison of genetically modified vibrios showed that flagella, an active toxR gene, and the virulence cassette were not essential for initial binding. Inactivation of the hemagglutinin/protease increased binding. This highly differentiated human intestinal cell line provides a versatile new approach for studying major events occurring during intestinal colonization: adherence, multiplication, and detachment.

Entities:  

Mesh：

Year:  1997        PMID： 9234816      PMCID： PMC175493          DOI： 10.1128/iai.65.8.3474-3477.1997

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  23 in total

1.  Lectin binding patterns to plasmalemmal glycoconjugates of goblet cells undergoing differentiation in vitro.

Authors:  E B Frisch; T E Phillips
Journal:  J Electron Microsc Tech       Date:  1990-09

2.  Adhesion of human enterotoxigenic Escherichia coli to human mucus secreting HT-29 cell subpopulations in culture.

Authors:  S Kerneis; M F Bernet; M H Coconnier; A L Servin
Journal:  Gut       Date:  1994-10       Impact factor: 23.059

3.  Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of an HA/protease-negative strain.

Authors:  C C Häse; R A Finkelstein
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

4.  Colonial opacity variations among the choleragenic vibrios.

Authors:  Richard A Finkelstein; Mary Boesman-Finkelstein; Dilip K Sengupta; William J Page; C Michael Stanley; Thomas E Phillips
Journal:  Microbiology (Reading)       Date:  1997-01       Impact factor: 2.777

5.  Adherence to human small intestines of capsulated Vibrio cholerae O139.

Authors:  T Yamamoto; M J Albert; R B Sack
Journal:  FEMS Microbiol Lett       Date:  1994-06-01       Impact factor: 2.742

6.  Vibrio cholerae adherence and colonization in experimental cholera: electron microscopic studies.

Authors:  E T Nelson; J D Clements; R A Finkelstein
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

7.  Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment.

Authors:  R A Finkelstein; M Boesman-Finkelstein; Y Chang; C C Häse
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

8.  Role of antibodies against biotype-specific Vibrio cholerae pili in protection against experimental classical and El Tor cholera.

Authors:  J Osek; G Jonson; A M Svennerholm; J Holmgren
Journal:  Infect Immun       Date:  1994-07       Impact factor: 3.441

9.  Cloning and sequencing of Vibrio cholerae mannose-sensitive haemagglutinin pilin gene: localization of mshA within a cluster of type 4 pilin genes.

Authors:  G Jonson; M Lebens; J Holmgren
Journal:  Mol Microbiol       Date:  1994-07       Impact factor: 3.501

10.  Toxin, toxin-coregulated pili, and the toxR regulon are essential for Vibrio cholerae pathogenesis in humans.

Authors:  D A Herrington; R H Hall; G Losonsky; J J Mekalanos; R K Taylor; M M Levine
Journal:  J Exp Med       Date:  1988-10-01       Impact factor: 14.307
View more
  38 in total

1.  Environmental signals controlling production of hemagglutinin/protease in Vibrio cholerae.

Authors:  J A Benitez; A J Silva; R A Finkelstein
Journal:  Infect Immun       Date:  2001-10       Impact factor: 3.441

2.  Attenuation of bacterial virulence by quorum sensing-regulated lysis.

Authors:  Anisia J Silva; Jorge A Benitez; Jian-He Wu
Journal:  J Biotechnol       Date:  2010-07-29       Impact factor: 3.307

3.  A novel dispersin protein in enteroaggregative Escherichia coli.

Authors:  Jalaluddin Sheikh; John R Czeczulin; Susan Harrington; Susan Hicks; Ian R Henderson; Chantal Le Bouguénec; Pierre Gounon; Alan Phillips; James P Nataro
Journal:  J Clin Invest       Date:  2002-11       Impact factor: 14.808

Review 4.  H-NS: an overarching regulator of the Vibrio cholerae life cycle.

Authors:  Julio C Ayala; Anisia J Silva; Jorge A Benitez
Journal:  Res Microbiol       Date:  2016-08-01       Impact factor: 3.992

Review 5.  Antibiofilm polysaccharides.

Authors:  Olaya Rendueles; Jeffrey B Kaplan; Jean-Marc Ghigo
Journal:  Environ Microbiol       Date:  2012-06-26       Impact factor: 5.491

6.  TcpF is a soluble colonization factor and protective antigen secreted by El Tor and classical O1 and O139 Vibrio cholerae serogroups.

Authors:  Thomas J Kirn; Ronald K Taylor
Journal:  Infect Immun       Date:  2005-08       Impact factor: 3.441

7.  Induction of interleukin-8 in T84 cells by Vibrio cholerae.

Authors:  Xin Zhou; Da Q Gao; Jane Michalski; Jorge A Benitez; James B Kaper
Journal:  Infect Immun       Date:  2004-01       Impact factor: 3.441

Review 8.  Vibrio cholerae hemagglutinin(HA)/protease: An extracellular metalloprotease with multiple pathogenic activities.

Authors:  Jorge A Benitez; Anisia J Silva
Journal:  Toxicon       Date:  2016-03-04       Impact factor: 3.033

9.  Homology modeling of hemagglutinin/protease [HA/P (vibriolysin)] from Vibrio cholerae: sequence comparision, residue interactions and molecular mechanism.

Authors:  Ghosia Lutfullah; Farhat Amin; Zahid Khan; Noreen Azhar; M Kamran Azim; Sajid Noor; Khalida Shoukat
Journal:  Protein J       Date:  2008-02       Impact factor: 2.371

Review 10.  Exploiting cholera vaccines as a versatile antigen delivery platform.

Authors:  Anisia J Silva; Francis O Eko; Jorge A Benitez
Journal:  Biotechnol Lett       Date:  2007-11-16       Impact factor: 2.461
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.