Literature DB >> 9234583

Differences in phycoerythrin- or fluorescein-isothiocyanate conjugated 8G12 on CD34+ cell evaluation.

F Ortuño1, F Ferrer, M L Lozano, I Heras, J M Moraleda, V Vicente.   

Abstract

To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.

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Year:  1997        PMID: 9234583

Source DB:  PubMed          Journal:  Haematologica        ISSN: 0390-6078            Impact factor:   9.941


  1 in total

1.  50nm-scale localization of single unmodified, isotopically enriched, proteins in cells.

Authors:  Anthony Delaune; Armelle Cabin-Flaman; Guillaume Legent; David Gibouin; Caroline Smet-Nocca; Fabrice Lefebvre; Arndt Benecke; Marc Vasse; Camille Ripoll
Journal:  PLoS One       Date:  2013-02-19       Impact factor: 3.240

  1 in total

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