| Literature DB >> 9234583 |
F Ortuño1, F Ferrer, M L Lozano, I Heras, J M Moraleda, V Vicente.
Abstract
To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.Entities:
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Year: 1997 PMID: 9234583
Source DB: PubMed Journal: Haematologica ISSN: 0390-6078 Impact factor: 9.941