| Literature DB >> 9234519 |
D R Sizemore1, A A Branstrom, J C Sadoff.
Abstract
Previously, Shigella carrier 15D was shown to deliver a mammalian DNA expression plasmid to cultured cells with subsequent production of the plasmid-encoded foreign protein. In this study, we report in vivo delivery of a DNA expression plasmid to mucosal tissue results in the stimulation of immune responses against the plasmid-encoded foreign antigen. Splenocytes from mice receiving two intranasal inoculations of 15D carrying pCMV beta showed proliferative responses to the plasmid-encoded Escherichia coli beta-galactosidase. In addition, antibody specific for beta-galactosidase was detected in pooled sera collected from 15D (pCMV beta) infected mice.Entities:
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Year: 1997 PMID： 9234519 DOI： 10.1016/s0264-410x(96)00252-6
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641