Reproducibility of shear rate and shear stress assessment by means of ultrasound in the common carotid artery of young human males and females.

In the present study, the reliability of an ultrasonic shear rate estimating system, in terms of intrasubject intrasession, intersubject intrasession and intersubject intersession variability coefficients for the assessment of wall shear rate (WSR) in the common carotid artery (CCA) was determined in eight presumed healthy volunteers. Measurements were performed on consecutive days (day 1, day 2 and day 7). To investigate whether there were differences in WSR due to gender, dynamic WSR in the CCA was assessed in 11 presumed healthy males (mean age 24 y) and 11 presumed healthy females (mean age 25 y). Wall shear stress (WSS) was estimated from WSR and calculated whole blood viscosity. The average intrasubject intrasession variability was about 15% for peak WSR and about 12% for mean WSR. The intersubject intrasession variability for peak WSR decreased from 19% on day 1 to 16% on day 7 and for mean WSR from 17% on day 1 to 11% on day 7. The intersubject intersession variability is on the order of 5% for peak WSR and about 4% for mean WSR. No significant differences could be detected between peak and mean WSR values on day 1, day 2 and day 7, indicating good short- and medium-term intersubject intersession reproducibilities. No differences in peak and mean WSR were found between the left and the right CCA in the male group as well as in the female group. Mean WSS was similar in males (1.3 +/- 0.3 Pa) and in females (1.2 +/- 0.2 Pa), but peak WSS was slightly, but significantly, higher in males (4.3 +/- 1.3 Pa) than in females (3.3 +/- 0.7 Pa). It can be concluded that peak and mean WSR can be reliably determined noninvasively using ultrasound.