Literature DB >> 9228677

Evaluation of a blocking Elisa for screening of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus.

K J Sørensen1, A Bøtner, E S Madsen, B Strandbygaard, J Nielsen.   

Abstract

A blocking Elisa was developed for the detection of antibodies against PRRS virus with a view to satisfying the need for examination of blood samples on a large scale. The test was evaluated in comparison with an indirect Elisa and the immunoperoxidase monolayer assay. The blocking Elisa was sensitive and specific. It had a higher capacity and was cheaper to perform than the immunoperoxidase monolayer assay and the indirect Elisa. It was comparable to the immunoperoxidase monolayer assay and better than the indirect Elisa in detecting antibodies formed early after infection, and it was superior to both the immunoperoxidase monolayer assay and the indirect Elisa in detecting antibodies at a late stage of infection.

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Year:  1997        PMID: 9228677     DOI: 10.1016/s0378-1135(96)01345-4

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN: 0378-1135            Impact factor:   3.293


  9 in total

1.  Nucleocapsid protein-based enzyme-linked immunosorbent assay for detection and differentiation of antibodies against European and North American porcine reproductive and respiratory syndrome virus.

Authors:  Torsten Seuberlich; Jon-Duri Tratschin; Barbara Thür; Martin A Hofmann
Journal:  Clin Diagn Lab Immunol       Date:  2002-11

2.  Validation of a blocking enzyme-linked immunosorbent assay for detection of antibodies against porcine reproductive and respiratory syndrome virus.

Authors:  Neal H Ferrin; Ying Fang; Craig R Johnson; Michael P Murtaugh; Dale D Polson; Montserrat Torremorell; Marie L Gramer; Eric A Nelson
Journal:  Clin Diagn Lab Immunol       Date:  2004-05

3.  Spatial analysis and temporal trends of porcine reproductive and respiratory syndrome in Denmark from 2007 to 2010 based on laboratory submission data.

Authors:  Ana Carolina Lopes Antunes; Tariq Halasa; Klara Tølbøl Lauritsen; Charlotte Sonne Kristensen; Lars Erik Larsen; Nils Toft
Journal:  BMC Vet Res       Date:  2015-12-21       Impact factor: 2.741

4.  Pathogenicity of three genetically diverse strains of PRRSV Type 1 in specific pathogen free pigs.

Authors:  Tomasz Stadejek; Lars E Larsen; Katarzyna Podgórska; Anette Bøtner; Sara Botti; Izabella Dolka; Michał Fabisiak; Peter M H Heegaard; Charlotte K Hjulsager; Tomasz Huć; Lise K Kvisgaard; Rafał Sapierzyński; Jens Nielsen
Journal:  Vet Microbiol       Date:  2017-05-16       Impact factor: 3.293

5.  Unweaving tangled mortality and antibiotic consumption data to detect disease outbreaks - Peaks, growths, and foresight in swine production.

Authors:  Ana Carolina Lopes Antunes; Vibeke Frøkjær Jensen; Dan Jensen
Journal:  PLoS One       Date:  2019-10-09       Impact factor: 3.240

6.  Opinion of the Scientific Panel on Animal Health and Welfare (AHAW) on a request from the Commission related to the probability of transmission of Porcine Reproductive and Respiratory Syndrome virus (PRRSv) to naive pigs via fresh meat.

Authors: 
Journal:  EFSA J       Date:  2005-08-12

7.  Experimental Infection of Young Pigs with an Early European Strain of Porcine Epidemic Diarrhoea Virus and a Recent US Strain.

Authors:  L Lohse; J S Krog; B Strandbygaard; T B Rasmussen; J Kjaer; G J Belsham; A Bøtner
Journal:  Transbound Emerg Dis       Date:  2016-05-10       Impact factor: 5.005

8.  Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus.

Authors:  Bertel Strandbygaard; Antonio Lavazza; Davide Lelli; Yannick Blanchard; Béatrice Grasland; Sophie Le Poder; Nicolas Rose; Falko Steinbach; Wim H M van der Poel; Frederik Widén; Graham J Belsham; Anette Bøtner
Journal:  Vet Microbiol       Date:  2016-11-23       Impact factor: 3.293

9.  Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes.

Authors:  M B Oleksiewicz; A Bøtner; K G Madsen; T Storgaard
Journal:  Vet Microbiol       Date:  1998-11       Impact factor: 3.293

  9 in total

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