Literature DB >> 9228484

Nonradioactive single-strand conformation polymorphism analysis for detection of fluoroquinolone resistance in mycobacteria.

W Sougakoff1, N Lemaître, E Cambau, M Szpytma, V Revel, V Jarlier.   

Abstract

A simple, rapid, and nonradioactive method for routine detection of fluoroquinolone resistance in mycobacteria is described. A single-strand conformation polymorphism (SSCP) methodology, based on the use of mini-gels and silver staining of DNA, was optimized for the analysis of denatured DNA products obtained by polymerase chain reaction (PCR) from the gyrA gene involved in fluoroquinolone resistance in mycobacteria. The method was successfully applied to fluoroquinolone-susceptible and -resistant laboratory strains of Mycobacterium smegmatis and to clinical strains of Mycobacterium tuberculosis isolated from patients who developed resistance during the course of fluoroquinolone treatment.

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Year:  1997        PMID： 9228484     DOI： 10.1007/bf01726372

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  17 in total

1.  Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

Authors:  M Orita; H Iwahana; H Kanazawa; K Hayashi; T Sekiya
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

Review 2.  Treatment of multidrug-resistant tuberculosis.

Authors:  M D Iseman
Journal:  N Engl J Med       Date:  1993-09-09       Impact factor: 91.245

3.  'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses.

Authors:  T Hongyo; G S Buzard; R J Calvert; C M Weghorst
Journal:  Nucleic Acids Res       Date:  1993-08-11       Impact factor: 16.971

4.  Detection of novel mutations in the gyrA gene of Staphylococcus aureus by nonradioisotopic single-strand conformation polymorphism analysis and direct DNA sequencing.

Authors:  Y Tokue; K Sugano; D Saito; T Noda; H Ohkura; Y Shimosato; T Sekiya
Journal:  Antimicrob Agents Chemother       Date:  1994-03       Impact factor: 5.191

5.  Selection of a gyrA mutant of Mycobacterium tuberculosis resistant to fluoroquinolones during treatment with ofloxacin.

Authors:  E Cambau; W Sougakoff; M Besson; C Truffot-Pernot; J Grosset; V Jarlier
Journal:  J Infect Dis       Date:  1994-08       Impact factor: 5.226

6.  Therapeutic effect of a new antibacterial substance ofloxacin (DL8280) on pulmonary tuberculosis.

Authors:  M Tsukamura; E Nakamura; S Yoshii; H Amano
Journal:  Am Rev Respir Dis       Date:  1985-03

7.  Characterization of mutations in Mycobacterium smegmatis involved in resistance to fluoroquinolones.

Authors:  V Revel; E Cambau; V Jarlier; W Sougakoff
Journal:  Antimicrob Agents Chemother       Date:  1994-09       Impact factor: 5.191

8.  A rapid method for the detection of potentially viable Mycobacterium leprae in human biopsies: a novel application of PCR.

Authors:  S A Woods; S T Cole
Journal:  FEMS Microbiol Lett       Date:  1989-12       Impact factor: 2.742

9.  Characterization of fluoroquinolone-resistant mutant strains of Mycobacterium tuberculosis selected in the laboratory and isolated from patients.

Authors:  G J Alangaden; E K Manavathu; S B Vakulenko; N M Zvonok; S A Lerner
Journal:  Antimicrob Agents Chemother       Date:  1995-08       Impact factor: 5.191

10.  Detection of gyrA and gyrB mutations in quinolone-resistant clinical isolates of Escherichia coli by single-strand conformational polymorphism analysis and determination of levels of resistance conferred by two different single gyrA mutations.

Authors:  S Ouabdesselam; D C Hooper; J Tankovic; C J Soussy
Journal:  Antimicrob Agents Chemother       Date:  1995-08       Impact factor: 5.191
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  4 in total

Review 1.  A systematic review of gyrase mutations associated with fluoroquinolone-resistant Mycobacterium tuberculosis and a proposed gyrase numbering system.

Authors:  Fernanda Maruri; Timothy R Sterling; Anne W Kaiga; Amondrea Blackman; Yuri F van der Heijden; Claudine Mayer; Emmanuelle Cambau; Alexandra Aubry
Journal:  J Antimicrob Chemother       Date:  2012-01-25       Impact factor: 5.790

2.  Rapid screening of fluoroquinolone resistance determinants in Streptococcus pneumoniae by PCR-restriction fragment length polymorphism and single-strand conformational polymorphism.

Authors:  Margaret Ip; Shirley S L Chau; Fang Chi; Amy Qi; Raymond W M Lai
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

3.  Evaluation of a new line probe assay for rapid identification of gyrA mutations in Mycobacterium tuberculosis.

Authors:  Federico Giannoni; Elisabetta Iona; Federica Sementilli; Lara Brunori; Manuela Pardini; Giovanni Battista Migliori; Graziella Orefici; Lanfranco Fattorini
Journal:  Antimicrob Agents Chemother       Date:  2005-07       Impact factor: 5.191

4.  Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates.

Authors:  Augustine F B Cheng; Wing W Yew; Edward W C Chan; Miu L Chin; Mamie M M Hui; Raphael C Y Chan
Journal:  Antimicrob Agents Chemother       Date:  2004-02       Impact factor: 5.191
  4 in total

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