PROBLEM: To investigate the proposal that NK cells in decidua may control trophoblast migration during implantation of the human placenta. METHOD: Use Mab specific for HLA-G and for HLA-C in association with flow cytometry and immunoprecipitation to determine the expression of these HLA molecules by trophoblast. Expression of Killer inhibitory/activatory receptors (KIR/KAR) and the CD94 receptor by decidual NK cells was also studied. RESULTS: Extravillous trophoblast expressed HLA-G and HLA-C in both beta2m-associated form and as free heavy chains. KIR and KAR are expressed by decidual NK cells. The repertoire of receptors varied between different women and also between blood and decidual NK cells from the same women. The expression of CD94 was also different between blood and decidual NK cells. CONCLUSION: The recognition of HLA-G/HLA-C by KIR/KAR and CD94 could provide a mechanism by which decidual NK cells control trophoblast migration.
PROBLEM: To investigate the proposal that NK cells in decidua may control trophoblast migration during implantation of the human placenta. METHOD: Use Mab specific for HLA-G and for HLA-C in association with flow cytometry and immunoprecipitation to determine the expression of these HLA molecules by trophoblast. Expression of Killer inhibitory/activatory receptors (KIR/KAR) and the CD94 receptor by decidual NK cells was also studied. RESULTS: Extravillous trophoblast expressed HLA-G and HLA-C in both beta2m-associated form and as free heavy chains. KIR and KAR are expressed by decidual NK cells. The repertoire of receptors varied between different women and also between blood and decidual NK cells from the same women. The expression of CD94 was also different between blood and decidual NK cells. CONCLUSION: The recognition of HLA-G/HLA-C by KIR/KAR and CD94 could provide a mechanism by which decidual NK cells control trophoblast migration.
Authors: P Paul; N Rouas-Freiss; I Khalil-Daher; P Moreau; B Riteau; F A Le Gal; M F Avril; J Dausset; J G Guillet; E D Carosella Journal: Proc Natl Acad Sci U S A Date: 1998-04-14 Impact factor: 11.205
Authors: Emily M Whettlock; Ee Von Woon; Antonia O Cuff; Brendan Browne; Mark R Johnson; Victoria Male Journal: Front Immunol Date: 2022-06-16 Impact factor: 8.786
Authors: Satarupa Basu; Patricia A Pioli; Jose Conejo-Garcia; Charles R Wira; Charles L Sentman Journal: Clin Immunol Date: 2008-08-26 Impact factor: 3.969
Authors: G Chaouat; E Menu; B Mognetti; M Moussa; V Cayol; Y Mostefaoui; S Dubanchet; P Khadel; J L Voluménie; C B Rongiéres; G L Delage Journal: Infect Dis Obstet Gynecol Date: 1997