Literature DB >> 9224602

Synthesis and hybridization properties of inverse oligonucleotides.

M Marangoni1, A Van Aerschot, P Augustyns, J Rozenski, P Herdewijn.   

Abstract

The synthesis of adenine and thymine cyclopentylethyl nucleosides is presented. This novel constrained monomeric building block is very difficult to incorporate into oligonucleotides. It was introduced in 13mer oligodeoxynucleotide sequences at a single position using H-phosphonate chemistry. Phosphoramidite chemistry completely failed in this particular case. The H-phosphonate building blocks were obtained starting from the corresponding phosphoramidites. Stability of duplexes with RNA and DNA is significantly reduced.

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Year:  1997        PMID： 9224602      PMCID： PMC146838          DOI： 10.1093/nar/25.15.3034

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  4 in total

1.  5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.

Authors:  J F Habener; C D Vo; D B Le; G P Gryan; L Ercolani; A H Wang
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

2.  Free energy of imperfect nucleic acid helices. 3. Small internal loops resulting from mismatches.

Authors:  J Gralla; D M Crothers
Journal:  J Mol Biol       Date:  1973-08-05       Impact factor: 5.469

3.  Hybridization specificity, enzymatic activity and biological (Ha-ras) activity of oligonucleotides containing 2,4-dideoxy-beta-D-erythro-hexopyranosyl nucleosides.

Authors:  K Augustyns; G Godard; C Hendrix; A Van Aerschot; J Rozenski; T Saison-Behmoaras; P Herdewijn
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

4.  5-Nitroindole as an universal base analogue.

Authors:  D Loakes; D M Brown
Journal:  Nucleic Acids Res       Date:  1994-10-11       Impact factor: 16.971
  4 in total
  1 in total

1.  Hybrid characteristics of oligonucleotides consisting of isonucleoside 2',5'-anhydro-3'-deoxy-3'-(thymin-1-yl)-D-mannitol with different linkage modes.

Authors:  Z Lei; L Zhang; L R Zhang; J Chen; J M Min; L H Zhang
Journal:  Nucleic Acids Res       Date:  2001-04-01       Impact factor: 16.971
  1 in total

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