Literature DB >> 9223530

A point mutation abolishes the helicase but not the nucleoside triphosphatase activity of hepatitis C virus NS3 protein.

G M Heilek1, M G Peterson.   

Abstract

The NS3 protein of hepatitis C virus contains a bipartite structure consisting of an N-terminal serine protease and a C-terminal DEAD box helicase. We show that the C-terminal domain has ATPase and panhelicase activities. The integrity of the helicase function is dependent on the conserved DEAD motif and can be abolished by a His-Ala point mutation, leaving a fully functional nucleoside triphosphatase.

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Year:  1997        PMID： 9223530      PMCID： PMC191896     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  17 in total

1.  Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.

Authors:  Q L Choo; G Kuo; A J Weiner; L R Overby; D W Bradley; M Houghton
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

2.  An improved assay for nanomole amounts of inorganic phosphate.

Authors:  P A Lanzetta; L J Alvarez; P S Reinach; O A Candia
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

3.  Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis.

Authors:  M Hijikata; N Kato; Y Ootsuyama; M Nakagawa; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

4.  Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide.

Authors:  J L Kim; K A Morgenstern; C Lin; T Fox; M D Dwyer; J A Landro; S P Chambers; W Markland; C A Lepre; E T O'Malley; S L Harbeson; C M Rice; M A Murcko; P R Caron; J A Thomson
Journal:  Cell       Date:  1996-10-18       Impact factor: 41.582

5.  Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.

Authors:  N Kato; M Hijikata; Y Ootsuyama; M Nakagawa; S Ohkoshi; T Sugimura; K Shimotohno
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

6.  The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site.

Authors:  R A Love; H E Parge; J A Wickersham; Z Hostomsky; N Habuka; E W Moomaw; T Adachi; Z Hostomska
Journal:  Cell       Date:  1996-10-18       Impact factor: 41.582

7.  The helicase activity associated with hepatitis C virus nonstructural protein 3 (NS3).

Authors:  C L Tai; W K Chi; D S Chen; L H Hwang
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

8.  Structure and organization of the hepatitis C virus genome isolated from human carriers.

Authors:  A Takamizawa; C Mori; I Fuke; S Manabe; S Murakami; J Fujita; E Onishi; T Andoh; I Yoshida; H Okayama
Journal:  J Virol       Date:  1991-03       Impact factor: 5.103

9.  Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites.

Authors:  A Grakoui; D W McCourt; C Wychowski; S M Feinstone; C M Rice
Journal:  J Virol       Date:  1993-05       Impact factor: 5.103

10.  Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.

Authors:  A E Gorbalenya; E V Koonin; A P Donchenko; V M Blinov
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
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  24 in total

1.  Mutations that affect dimer formation and helicase activity of the hepatitis C virus helicase.

Authors:  Y L Khu; E Koh; S P Lim; Y H Tan; S Brenner; S G Lim; W J Hong; P Y Goh
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

2.  RNA helicase dynamics in pre-mRNA splicing.

Authors:  B Schwer; T Meszaros
Journal:  EMBO J       Date:  2000-12-01       Impact factor: 11.598

3.  Mutagenesis of the Dengue virus type 2 NS3 protein within and outside helicase motifs: effects on enzyme activity and virus replication.

Authors:  A E Matusan; M J Pryor; A D Davidson; P J Wright
Journal:  J Virol       Date:  2001-10       Impact factor: 5.103

4.  ATP-dependent remodeling of the spliceosome: intragenic suppressors of release-defective mutants of Saccharomyces cerevisiae Prp22.

Authors:  Eva Campodonico; Beate Schwer
Journal:  Genetics       Date:  2002-02       Impact factor: 4.562

5.  The nonstructural protein 3 protease/helicase requires an intact protease domain to unwind duplex RNA efficiently.

Authors:  David N Frick; Ryan S Rypma; Angela M I Lam; Baohua Gu
Journal:  J Biol Chem       Date:  2003-10-29       Impact factor: 5.157

6.  Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A.

Authors:  Ting Xu; Aruna Sampath; Alex Chao; Daying Wen; Max Nanao; Patrick Chene; Subhash G Vasudevan; Julien Lescar
Journal:  J Virol       Date:  2005-08       Impact factor: 5.103

7.  Three conformational snapshots of the hepatitis C virus NS3 helicase reveal a ratchet translocation mechanism.

Authors:  Meigang Gu; Charles M Rice
Journal:  Proc Natl Acad Sci U S A       Date:  2009-12-31       Impact factor: 11.205

8.  The RNA helicase and nucleotide triphosphatase activities of the bovine viral diarrhea virus NS3 protein are essential for viral replication.

Authors:  B Gu; C Liu; J Lin-Goerke; D R Maley; L L Gutshall; C A Feltenberger; A M Del Vecchio
Journal:  J Virol       Date:  2000-02       Impact factor: 5.103

9.  A nucleotide-dependent and HRDC domain-dependent structural transition in DNA-bound RecQ helicase.

Authors:  Zsuzsa S Kocsis; Kata Sarlós; Gábor M Harami; Máté Martina; Mihály Kovács
Journal:  J Biol Chem       Date:  2014-01-08       Impact factor: 5.157

10.  Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family.

Authors:  A Martins; C H Gross; S Shuman
Journal:  J Virol       Date:  1999-02       Impact factor: 5.103
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